B3GY37 (SYD_ACTP7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	APP7_1208

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_1000090952

Sequences

	Sequence		Length	Mass (Da)
	B3GY37 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 1A2502B3F062FE44	FASTA	591	66,810
	10 20 30 40 50 60 MMRSHYCGAL NRSHVGQTVT LSGWVHRVRN LGRFIFMQIR DREGIVQVFF DEKDEAIFKI 70 80 90 100 110 120 ASSLRSEACV QIQGEVIARD ESQINKEMAT GEIEVLVKNV VVYNNADVLP LDFNQNNTEE 130 140 150 160 170 180 QRLKYRYLDL RRPEMAEKLK TRAKITSFVR RYMDDNGFLD IETPMLTKAT PEGARDYLVP 190 200 210 220 230 240 SRVHNGKFYA LPQSPQLFKQ LLMMSGFDRY YQIVKCFRDE DLRADRQPEF TQIDVETSFL 250 260 270 280 290 300 TAEEVRELME NMIHGLWLDR LNVDLGKFPI MTWQEAMQRF GSDKPDLRNP LELVDVADIL 310 320 330 340 350 360 KDVEFKVFNE PANSADGRVT VLRVPNGASL TRKQIDEYTQ FVGIYGAKGL AWAKINDVNA 370 380 390 400 410 420 GMEGIQSPVA KFLNEEVFKA LIERTNATSG DILFFGADKW QVVTDSMGAL RLKVGRDLAL 430 440 450 460 470 480 TDLSAWKPLW VIDFPMFEKD DEGNLSAMHH PFTSPKNLTP EELAANPVNA VANAYDMVIN 490 500 510 520 530 540 GYEVGGGSVR IYDPKMQQTV FGILGINEQD QQEKFGFLLD ALKFGTPPHA GLAFGLDRLT 550 560 570 580 590 MLITGTENIR DVIAFPKTTA AACLMTEAPS FANPQALEEL GIAVLKKEKA E

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AP76.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001091 Genomic DNA. Translation: ACE61860.1.
RefSeq	YP_001969002.1. NC_010939.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B3GY37.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6395870.
GenomeReviews	Gene locus APP7_1208 in contig CP001091_GR.
KEGG	apa:APP7_1208.
PATRIC	20756073. VBIActPle116979_1234.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG396032.
OMA	AFPKTQQ.
ProtClustDB	PRK00476.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_ACTP7

Accession

Primary (citable) accession number: B3GY37

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program