B3GXZ9 (RLMC_ACTP7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
23S rRNA (uracil(747)-C(5))-methyltransferase RlmC
EC=2.1.1.189

Alternative name(s):
23S rRNA(m5U747)-methyltransferase

Gene names

Name:	rlmC
Ordered Locus Names:	APP7_1170

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus › Actinobacillus pleuropneumoniae › Actinobacillus pleuropneumoniae serovar 7

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 5-methyl-uridine at position 747 (m5U747) in 23S rRNA By similarity. HAMAP-Rule MF_01012
Catalytic activity	S-adenosyl-L-methionine + uracil(747) in 23S rRNA = S-adenosyl-L-homocysteine + 5-methyluracil(747) in 23S rRNA. HAMAP-Rule MF_01012
Sequence similarities	Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RlmC subfamily.

Ontologies

Keywords
Biological process	rRNA processing
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: HAMAP rRNA (uridine-C5-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 391	391	23S rRNA (uracil(747)-C(5))-methyltransferase RlmC HAMAP-Rule MF_01012		PRO_0000414816
Sites
	Active site	349	1	Nucleophile By similarity
	Metal binding	5	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	13	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	16	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	95	1	Iron-sulfur (4Fe-4S) By similarity
	Binding site	220	1	S-adenosyl-L-methionine By similarity
	Binding site	249	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	276	1	S-adenosyl-L-methionine By similarity
	Binding site	322	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3GXZ9 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 7D8A70C03AB41A99	FASTA	391	44,167
	10 20 30 40 50 60 MILNCPHFQQ QDCVSCQWLE KPYATQLTDK EIDLKRLISP FILQNFTEIL PPVQSSQKQF 70 80 90 100 110 120 RNKAKMVVSG SVERPILGIL KDQTDPQSGI DLCDCPLYPT EFEALFPILK DFIARAGLVP 130 140 150 160 170 180 YNIAKKKGEL KYILLTQSRY NQSIMLRFVL KSEQKRPLVE RELPNLLAKL PKDSVVSLNI 190 200 210 220 230 240 QPQHAAILEG ETEIFLTEKT TIEENFNDIP LFIRPQGFFQ TNPNVASQLY ATAQNWIKDL 250 260 270 280 290 300 AIQQFWDLFC GVGGFGLHCA KALQEKNENV QLTSIEISAS AIASATKSAE QLQLKNITFA 310 320 330 340 350 360 SLDSAQFALN EKSATPDLVI VNPPRRGIGK PLAEFLNQLG TPYLIYSSCN ARTMAQDFEA 370 380 390 LSNYSLQKVQ LFDMFPHTSH YEVLTFLVKK S

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AP76.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001091 Genomic DNA. Translation: ACE61822.1.
RefSeq	YP_001968964.1. NC_010939.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	537457.APP7_1170.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACE61822; ACE61822; APP7_1170.
GeneID	6397907.
KEGG	apa:APP7_1170.
PATRIC	20755987. VBIActPle116979_1195.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2265.
HOGENOM	HOG000218547.
KO	K03212.
OMA	QPQHAAI.
ProtClustDB	PRK03522.
Enzyme and pathway databases
BioCyc	APLE537457:GJI0-1192-MONOMER.
Family and domain databases
HAMAP	MF_01012. 23SrRNA_methyltr_RlmC.
InterPro	IPR011825. 23SrRNA_MeTrfase_RlmC. IPR010280. U5_MeTrfase. [Graphical view]
Pfam	PF05958. tRNA_U5-meth_tr. 1 hit. [Graphical view]
TIGRFAMs	TIGR02085. meth_trns_rumB. 1 hit.
PROSITE	PS01230. TRMA_1. 1 hit. PS01231. TRMA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RLMC_ACTP7

Accession

Primary (citable) accession number: B3GXZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 25, 2012
Last sequence update:	July 22, 2008
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program