Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B3GXZ7 (NAGZ_ACTP7) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-hexosaminidase

EC=3.2.1.52
Alternative name(s):
Beta-N-acetylhexosaminidase
N-acetyl-beta-glucosaminidase
Gene names
Name:nagZ
Ordered Locus Names:APP7_1168
OrganismActinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]
Taxonomic identifier537457 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeActinobacillus

Protein attributes

Sequence length345 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides By similarity. HAMAP-Rule MF_00364

Catalytic activity

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP-Rule MF_00364

Pathway

Cell wall biogenesis; peptidoglycan recycling. HAMAP-Rule MF_00364

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00364.

Sequence similarities

Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 345345Beta-hexosaminidase HAMAP-Rule MF_00364
PRO_1000121051

Regions

Region162 – 1632Substrate binding By similarity

Sites

Active site1751Proton donor/acceptor By similarity
Active site2471Nucleophile By similarity
Binding site601Substrate By similarity
Binding site681Substrate By similarity
Binding site1321Substrate By similarity
Site1731Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
B3GXZ7 [UniParc].

Last modified July 22, 2008. Version 1.
Checksum: AB16DBBA7B4F3CDD

FASTA34538,994
        10         20         30         40         50         60 
MLLIDIKDKE LSQEEVEILE HPLVSGLILF SRNFHDKVQL EALVKSIRQR VKKPLLITVD 

        70         80         90        100        110        120 
QEGGRVQRFR EGFTKLPAMQ AFHTLAKNPQ ESTALARQTG WLMAAEMFAL DIDLSFAPVL 

       130        140        150        160        170        180 
DLGHQCKAIG DRSFGENPDA MLPIAEAFID GMREMGMATT GKHFPGHGHV LADSHLETPF 

       190        200        210        220        230        240 
DDRPKEAIFN RDILPFKQLI SKGKLSAIMP AHVIYTQCDS QPASGSEYWL KQVLRNQLNF 

       250        260        270        280        290        300 
NGVIFSDDLG MKGAGFMGNF VERSEKAIHA GCDLLLLCNE PEGVIQVLDG LKYQPTKTQT 

       310        320        330        340 
ERHISLMKRK TVRWNELEAS PRYQQAQQRL TALQNDWLEY KAQHC 

« Hide

References

[1]"Genome and proteome analysis of A. pleuropneumoniae serotype 7."
Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F.
Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AP76.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001091 Genomic DNA. Translation: ACE61820.1.
RefSeqYP_001968962.1. NC_010939.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING537457.APP7_1168.

Protein family/group databases

CAZyGH3. Glycoside Hydrolase Family 3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACE61820; ACE61820; APP7_1168.
GeneID6397914.
KEGGapa:APP7_1168.
PATRIC20755983. VBIActPle116979_1193.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1472.
HOGENOMHOG000248526.
KOK01207.
OMAHILALYE.
OrthoDBEOG6BCT06.
ProtClustDBPRK05337.

Enzyme and pathway databases

BioCycAPLE537457:GJI0-1190-MONOMER.
UniPathwayUPA00544.

Family and domain databases

Gene3D3.20.20.300. 1 hit.
HAMAPMF_00364. NagZ.
InterProIPR022956. Beta_hexosaminidase_bac.
IPR019800. Glyco_hydro_3_AS.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAGZ_ACTP7
AccessionPrimary (citable) accession number: B3GXZ7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 22, 2008
Last modified: April 16, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries