Reviewed, UniProtKB/Swiss-Prot B3GXR0 (SYC_ACTP7)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	APP7_0973

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	Cysteinyl-tRNA synthetase HAMAP MF_00041		PRO_1000090812
Regions
	Motif	30 – 40	11	"HIGH" region HAMAP MF_00041
	Motif	266 – 270	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	28	1	Zinc By similarity
	Metal binding	209	1	Zinc By similarity
	Metal binding	234	1	Zinc By similarity
	Metal binding	238	1	Zinc By similarity
	Binding site	269	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3GXR0-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: AE45A40EA1256B9B	FASTA	459	52,154
	10 20 30 40 50 60 MLKIYNTLKR EKEEFKPINS NQVGMYVCGV TVYDLCHFGH GRTFVSFDVI ARYLRYLGYN 70 80 90 100 110 120 LRYVRNITDV DDKIIKRALE NNEMCDQLVD RMIAEMHKDF DDLNILRPDV EPRATKHIPE 130 140 150 160 170 180 IVAMVEKLIA NGHAYVAADG DVMFDVESFK KYGALSRQNL EQLQAGARVE IKSVKKNPMD 190 200 210 220 230 240 FVLWKMSKEG EPSWQSPWGN GRPGWHIECS AMNSKELGEH FDIHGGGSDL MFPHHENEIA 250 260 270 280 290 300 QSCCAHGGDY VNYWLHTGML TIDDEKMSKS LGNFFTIRTM LEKYESETLR YFFLTAHYRS 310 320 330 340 350 360 LLNYSLNNLD LARSALERLY TSLRGCDLSV EVAGGEQYVE AFKAAMDDDF NTPGALAVLF 370 380 390 400 410 420 EIAREVNKLK TEDMAKANGL AARLKELAGV LGLLYQDPEA FLQGDADNDE VAEIEALIKQ 430 440 450 RNEAKAAKNW AVADEVRDKL KAMNIVLEDT PNGTTWRKA

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001091 Genomic DNA. Translation: ACE61625.1.
RefSeq	YP_001968767.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6398025.
GenomeReviews	Gene locus APP7_0973 in contig CP001091_GR.
KEGG	apa:APP7_0973.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VLWKAAK.
Family and domain databases
HAMAP	MF_00041. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_ACTP7

Accession

Primary (citable) accession number: B3GXR0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)