B3GXD3 (ARGA_ACTP7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Amino-acid acetyltransferase
EC=2.3.1.1

Alternative name(s):
N-acetylglutamate synthase
Short name=AGS
Short name=NAGS

Gene names

Name:	argA
Ordered Locus Names:	APP7_0740

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	437 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01105
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01105
Subcellular location	Cytoplasm By similarity HAMAP MF_01105.
Miscellaneous	In bacteria which possess the bifunctional enzyme ornithine acetyltransferase/N-acetylglutamate synthase (ArgJ), ArgA fulfills an anaplerotic role. HAMAP MF_01105
Sequence similarities	Belongs to the acetyltransferase family. ArgA subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA:L-glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 437	437	Amino-acid acetyltransferase HAMAP MF_01105		PRO_1000137048
Regions
	Domain	289 – 429	141	N-acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	B3GXD3 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 11F30BAC50AB9F1E	FASTA	437	49,336
	10 20 30 40 50 60 MRNTELVQWF RQSTPYVNMH REKTFVIMLD GNAIAHPNFI NITNDISLLH SLGIKLVIVF 70 80 90 100 110 120 GARCQIDELL AKNQMSSTYH KHIRITDSKT LEVVKQAVGG LHYDIFSRLS LRLPNSPVLN 130 140 150 160 170 180 VVSSNAVLAQ PLGVIDGVDY GLSGKIRRIN IEAIQQQLAQ DAIVVIGPIA PSVTGEMFNL 190 200 210 220 230 240 PFEEIATQIA IKLKADKLIG FCDQQGILDS EGNVLSDLHP REAKRYLTQF IESGQYHHSA 250 260 270 280 290 300 ARFLQAAIEA CHAGIKRSHL LSYKEDGSLL QELFSRDGIG TQLSEESSEN IRLATSFDIP 310 320 330 340 350 360 GLLNLIRPLE EQGILVKRSR EQLEMEISNY TIIERDGIVI ACAALNHYPQ EKMAEMACVA 370 380 390 400 410 420 VHPDYRDSSR GDVLLEAIKR RAYKLQVEKL FVLTTRTTQW FQERGFVLST TDDLPKEKRE 430 HYNYQRMSKI LILDLGQ

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AP76.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001091 Genomic DNA. Translation: ACE61392.1.
RefSeq	YP_001968534.1. NC_010939.1.
3D structure databases
ProteinModelPortal	B3GXD3.
ModBase	Search...
Protein-protein interaction databases
STRING	B3GXD3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6396198.
GenomeReviews	Gene locus APP7_0740 in contig CP001091_GR.
KEGG	apa:APP7_0740.
PATRIC	20755101. VBIActPle116979_0761.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG545264.
OMA	AFNLAME.
ProtClustDB	PRK05279.
Family and domain databases
HAMAP	MF_01105. N-acetyl_glu_synth. [Tree]
InterPro	IPR000182. AcTrfase_GCN5-related_dom. IPR016181. Acyl_CoA_acyltransferase. IPR001048. Asp/Glu/Uridylate_kinase. IPR010167. NH2A_AcTrfase_ArgA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
KO	K14682.
Pfam	PF00696. AA_kinase. 1 hit. PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PIRSF	PIRSF000423. ArgA. 1 hit.
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF55729. Acyl_CoA_acyltransferase. 1 hit.
TIGRFAMs	TIGR01890. N-Ac-Glu-synth. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGA_ACTP7

Accession

Primary (citable) accession number: B3GXD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program