Reviewed, UniProtKB/Swiss-Prot B3GX43 (FTHS_ACTP7)

Names and origin

Protein names

Recommended name:
    Formate--tetrahydrofolate ligase
    EC=6.3.4.3
Alternative name(s):
    Formyltetrahydrofolate synthetase
      Short name=FHS
      Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	APP7_0543

Organism

Actinobacillus pleuropneumoniae serotype 7 (strain AP76) [Complete proteome] [HAMAP]

Taxonomic identifier

537457 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Actinobacillus

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Formate--tetrahydrofolate ligase HAMAP MF_01543		PRO_1000146670
Regions
	Nucleotide binding	64 – 71	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3GX43-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: AD5278FBF34F08E5	FASTA	556	58,955
	10 20 30 40 50 60 MKSDVEIAQA ATMQPIHKIA EKLGLNADQI EQYGKYKAKI NPTDAFKLPA KNGKLILVTA 70 80 90 100 110 120 INPTPAGEGK TTVTIGLTDA LNQLGKNAVV AAREPSLGPV FGVKGGAAGG GYAQVLPMED 130 140 150 160 170 180 INLHFTGDFH AITSANNLLA ALLDNHIYQG NALNIDTKRV LWRRVIDMND RQLRNVLGGL 190 200 210 220 230 240 GNPTDGVIRP DGFDITVASE VMAIFCLAKD LADLKTRLGN ILVAYTKDKQ PVYAKDLNAH 250 260 270 280 290 300 GAMAALLKDA IKPNLVQTIE GSPAFIHGGP FANIAHGCNS VTATRLALHL GDYAVTEAGF 310 320 330 340 350 360 GADLGAEKFC DIKCRLADLK PDVAVVVATV KALKYNGGVE KANLAEENLT ALQQGLPNLL 370 380 390 400 410 420 KHISNLKNVF GLPVVVALNR FVSDTDAELA LIQTACAKQG VEVSLTEVWG KGGAGGVDLA 430 440 450 460 470 480 QKVLKAIDEQ ENRFNFVYDV NESVQNKIKA IAQKIYGADD VNFSAEALAE IKNLEKLGLD 490 500 510 520 530 540 KLPICMAKTQ YSLSDNAKLL GCPSGFTVTV RSISVSAGAG FIVAICGSIM RMPGLPKVPA 550 ANRIDVDENG LITGLF

References

[1]	"Genome and proteome analysis of A. pleuropneumoniae serotype 7." Linke B., Buettner F., Martinez-Arias R., Goesmann A., Baltes N., Tegetmeyer H., Singh M., Gerlach G.F. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001091 Genomic DNA. Translation: ACE61195.1.
RefSeq	YP_001968337.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6396558.
GenomeReviews	Gene locus APP7_0543 in contig CP001091_GR.
KEGG	apa:APP7_0543.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KMPGLPK.
Family and domain databases
HAMAP	MF_01543. [Tree]
InterPro	IPR000559. For_THF_ligase. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_ACTP7

Accession

Primary (citable) accession number: B3GX43

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 22, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)