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Protein

Acetylserotonin O-methyltransferase

Gene

Asmt

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine).By similarity

Miscellaneous

Pineal melatonin synthesis is severely compromised in most inbred strains. In many inbred strains, genetic defects in ASMT have been identified. Melatonin production may have an impact on gonadal development, testis development being significantly promoted in melatonin-deficient C57BL/6J x Mus musculus molossinus animals.

Catalytic activityi

S-adenosyl-L-methionine + N-acetylserotonin = S-adenosyl-L-homocysteine + melatonin.

Pathwayi: melatonin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes melatonin from serotonin.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Acetylserotonin O-methyltransferase (Asmt), Serotonin N-acetyltransferase (Aanat)
  2. no protein annotated in this organism
This subpathway is part of the pathway melatonin biosynthesis, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes melatonin from serotonin, the pathway melatonin biosynthesis and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei146S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei163S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei209S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei252S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei255Proton donor/acceptorBy similarity1
Binding sitei256SubstrateBy similarity1
Binding sitei302SubstrateBy similarity1
Binding sitei306SubstrateBy similarity1

GO - Molecular functioni

  • acetylserotonin O-methyltransferase activity Source: RGD
  • S-adenosylmethionine-dependent methyltransferase activity Source: RGD

GO - Biological processi

  • melatonin biosynthetic process Source: RGD

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processMelatonin biosynthesis
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-RNO-209931 Serotonin and melatonin biosynthesis
UniPathwayiUPA00837; UER00815

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylserotonin O-methyltransferase (EC:2.1.1.4)
Alternative name(s):
Hydroxyindole O-methyltransferase
Gene namesi
Name:Asmt
Synonyms:Hiomt
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi708472 Asmt

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004147961 – 432Acetylserotonin O-methyltransferaseAdd BLAST432

Proteomic databases

PaxDbiB3GSH5
PRIDEiB3GSH5

Expressioni

Tissue specificityi

Expressed predominantly in the pineal gland (at protein level). Very low expression, if any, in the retina.2 Publications

Inductioni

Exhibits very slight night/day variation, if any.1 Publication

Gene expression databases

BgeeiENSRNOG00000001324
GenevisibleiB3GSH5 RN

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001791

Structurei

3D structure databases

ProteinModelPortaliB3GSH5
SMRiB3GSH5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni235 – 237S-adenosyl-L-methionine bindingPROSITE-ProRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3178 Eukaryota
ENOG410XS7T LUCA
GeneTreeiENSGT00530000064032
HOGENOMiHOG000247024
HOVERGENiHBG001526
InParanoidiB3GSH5
KOiK00543
OMAiFWPYVFG
OrthoDBiEOG091G0PPM

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR031725 ASMT_dimerisation
IPR016461 O-MeTrfase_COMT
IPR001077 O_MeTrfase_2
IPR029063 SAM-dependent_MTases
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF16864 Dimerisation2, 1 hit
PF00891 Methyltransf_2, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51683 SAM_OMT_II, 1 hit

Sequencei

Sequence statusi: Complete.

B3GSH5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPGREGELD RDFRVLMSLA HGFMVSQVLF AALDLGIFDL AAQGPVAAEA
60 70 80 90 100
VAQTGGWSPR GTQLLMDACT RLGLLRGAGD GSYTNSALSS TFLVSGSPQS
110 120 130 140 150
QRCMLLYLAG TTYGCWAHLA AGVREGRNQY SRAVGISAED PFSAIYRSEP
160 170 180 190 200
ERLLFMRGLQ ETWSLCGGRV LTAFDLSRFR VICDLGGGSG ALAQEAARLY
210 220 230 240 250
PGSSVCVFDL PDVIAAARTH FLSPGARPSV RFVAGDFFRS RLPRADLFIL
260 270 280 290 300
ARVLHDWADG ACVELLGRLH RACRPGGALL LVEAVLAKGG AGPLRSLLLS
310 320 330 340 350
LNMMLQAEGW ERQASDYRNL ATRAGFPRLQ LRRPGGPYHA MLARRGPRPG
360 370 380 390 400
IITGVGSNTT GTGSFVTGIR RDVPGARSDA AGTGSGTGNT GSGIMLQGET
410 420 430
LESEVSAPQA GSDVGGAGNE PRSGTLKQGD WK
Length:432
Mass (Da):45,588
Last modified:July 22, 2008 - v1
Checksum:i0F782CD0AB6293F5
GO

Sequence cautioni

The sequence AAB61243 differs from that shown. Reason: Frameshift at several positions.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L78306 mRNA Translation: AAB61243.1 Frameshift.
EU741665 mRNA Translation: ACD87748.1
CH474012 Genomic DNA Translation: EDL89812.1
RefSeqiNP_653360.2, NM_144759.2
UniGeneiRn.10768

Genome annotation databases

EnsembliENSRNOT00000001791; ENSRNOP00000001791; ENSRNOG00000001324
GeneIDi246281
KEGGirno:246281
UCSCiRGD:708472 rat

Entry informationi

Entry nameiASMT_RAT
AccessioniPrimary (citable) accession number: B3GSH5
Secondary accession number(s): O09179
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: July 22, 2008
Last modified: May 23, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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