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Protein

G-protein coupled estrogen receptor 1

Gene

gper1

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Membrane G-protein coupled estrogen receptor that binds to 17-beta-estradiol (E2) with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways. Plays a role in the embryonic development of sensory and motor neurons. Specifically induces apoptosis and reduces proliferation of brain cells. Involved in maintenance of meiotic arrest in oocytes.3 Publications

Kineticsi

Binds 17-beta-estradiol (E2) in plasma membranes with high affinity and displays rapid kinetics of association and dissociation.1 Publication

      GO - Molecular functioni

      • estrogen receptor activity Source: ZFIN
      • G-protein coupled receptor activity Source: UniProtKB-KW
      • steroid binding Source: UniProtKB
      • steroid hormone binding Source: UniProtKB

      GO - Biological processi

      • apoptotic process Source: UniProtKB-KW
      • brain development Source: UniProtKB
      • cell cycle Source: UniProtKB-KW
      • cell differentiation Source: UniProtKB-KW
      • cellular response to estradiol stimulus Source: UniProtKB
      • negative regulation of meiotic cell cycle Source: UniProtKB
      • negative regulation of neuron apoptotic process Source: UniProtKB
      • negative regulation of oocyte maturation Source: UniProtKB
      • positive regulation of cAMP-mediated signaling Source: UniProtKB
      • positive regulation of embryonic development Source: UniProtKB
      • positive regulation of neural precursor cell proliferation Source: UniProtKB
      • positive regulation of protein phosphorylation Source: UniProtKB
      • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
      • steroid hormone mediated signaling pathway Source: GOC
      Complete GO annotation...

      Keywords - Molecular functioni

      Developmental protein, G-protein coupled receptor, Receptor, Transducer

      Keywords - Biological processi

      Apoptosis, Cell cycle, Differentiation, Neurogenesis

      Enzyme and pathway databases

      ReactomeiR-DRE-375276. Peptide ligand-binding receptors.
      R-DRE-418594. G alpha (i) signalling events.

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      G-protein coupled estrogen receptor 1
      Alternative name(s):
      G protein-coupled estrogen receptor 1
      G-protein coupled receptor 30
      Gene namesi
      Name:gper1
      OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
      Taxonomic identifieri7955 [NCBI]
      Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
      Proteomesi
      • UP000000437 Componenti: Chromosome 3

      Organism-specific databases

      ZFINiZDB-GENE-090311-1. gper1.

      Subcellular locationi

      Topology

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Topological domaini1 – 4040ExtracellularSequence analysisAdd
      BLAST
      Transmembranei41 – 6121Helical; Name=1Sequence analysisAdd
      BLAST
      Topological domaini62 – 8120CytoplasmicSequence analysisAdd
      BLAST
      Transmembranei82 – 10221Helical; Name=2Sequence analysisAdd
      BLAST
      Topological domaini103 – 11210ExtracellularSequence analysis
      Transmembranei113 – 13321Helical; Name=3Sequence analysisAdd
      BLAST
      Topological domaini134 – 16027CytoplasmicSequence analysisAdd
      BLAST
      Transmembranei161 – 18121Helical; Name=4Sequence analysisAdd
      BLAST
      Topological domaini182 – 20221ExtracellularSequence analysisAdd
      BLAST
      Transmembranei203 – 22321Helical; Name=5Sequence analysisAdd
      BLAST
      Topological domaini224 – 24522CytoplasmicSequence analysisAdd
      BLAST
      Transmembranei246 – 26621Helical; Name=6Sequence analysisAdd
      BLAST
      Topological domaini267 – 29226ExtracellularSequence analysisAdd
      BLAST
      Transmembranei293 – 31321Helical; Name=7Sequence analysisAdd
      BLAST
      Topological domaini314 – 35340CytoplasmicSequence analysisAdd
      BLAST

      GO - Cellular componenti

      Complete GO annotation...

      Keywords - Cellular componenti

      Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Mitochondrion, Nucleus, Postsynaptic cell membrane, Synapse

      Pathology & Biotechi

      Disruption phenotypei

      Morpholino knockdown of the protein leads to growth retardation and developmental deformity.1 Publication

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Chaini1 – 353353G-protein coupled estrogen receptor 1PRO_0000424544Add
      BLAST

      Amino acid modifications

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Disulfide bondi115 ↔ 192PROSITE-ProRule annotation

      Keywords - PTMi

      Disulfide bond

      Proteomic databases

      PaxDbiB3G515.

      Expressioni

      Tissue specificityi

      Expressed in brain regions that are known to control reproduction and sex behavior. Expressed in ovary, muscle and intestine. Expressed in early germ cells of the testis, including the spermatogonia, spermatocytes, and somatic cells such as Sertoli cells.1 Publication

      Developmental stagei

      Expressed throughout early embryonic development from 0 hours post-fertilization (hpf) to 72 hpf. Expressed in blastomeres at 4 hpf. Expressed in the central nervous system at 18 hpf. Expressed in head including the anterior diencephalon, midbrain and hindbrain at 24 hpf. Expressed in trigeminal ganglia as well as in the heart, pancreas and intestinal bulb between 36 and 72 hpf (at protein level).1 Publication

      Interactioni

      Subunit structurei

      Homodimer. Heterodimer (By similarity).By similarity

      Protein-protein interaction databases

      STRINGi7955.ENSDARP00000070486.

      Structurei

      3D structure databases

      ProteinModelPortaliB3G515.
      ModBaseiSearch...
      MobiDBiSearch...

      Family & Domainsi

      Sequence similaritiesi

      Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

      Keywords - Domaini

      Transmembrane, Transmembrane helix

      Phylogenomic databases

      eggNOGiENOG410IEGB. Eukaryota.
      ENOG4111653. LUCA.
      GeneTreeiENSGT00530000063910.
      HOGENOMiHOG000013114.
      HOVERGENiHBG005351.
      InParanoidiB3G515.
      KOiK04246.
      OMAiQHARLSC.
      OrthoDBiEOG7WX08J.
      PhylomeDBiB3G515.
      TreeFamiTF333506.

      Family and domain databases

      InterProiIPR000276. GPCR_Rhodpsn.
      IPR017452. GPCR_Rhodpsn_7TM.
      [Graphical view]
      PfamiPF00001. 7tm_1. 1 hit.
      [Graphical view]
      PRINTSiPR00237. GPCRRHODOPSN.
      PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
      PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
      [Graphical view]

      Sequencei

      Sequence statusi: Complete.

      B3G515-1 [UniParc]FASTAAdd to basket

      « Hide

              10         20         30         40         50
      MEEQTTNVIQ IYVNGTEQFN ASFDFNITDV KESTDTYEFY IIGLFLSCLY
      60 70 80 90 100
      TIFLFPIGFI GNILILVVNL NHRERMTIPD LYFVNLAVAD LILVADSLIE
      110 120 130 140 150
      VFNLNEKYYD YAVLCTFMSL FLQVNMYSSI FFLTWMSFDR YVALTSSMSS
      160 170 180 190 200
      SPLRTMQHAK LSCSLIWMAS ILATLLPFTI VQTQHTGEVH FCFANVFEIQ
      210 220 230 240 250
      WLEVTIGFLI PFSIIGLCYS LIVRTLMRAQ KHKGLWPRRQ KALRMIVVVV
      260 270 280 290 300
      LVFFICWLPE NVFISIQLLQ GTADPSKRTD TTLWHDYPLT GHIVNLAAFS
      310 320 330 340 350
      NSCLNPIIYS FLGETFRDKL RLFIKRKASW SVVYRFCNHT LDLQIPVRSE

      SEV
      Length:353
      Mass (Da):40,859
      Last modified:July 22, 2008 - v1
      Checksum:iB88913B13954D4B2
      GO

      Sequence databases

      Select the link destinations:
      EMBLi
      GenBanki
      DDBJi
      Links Updated
      EU652771 mRNA. Translation: ACD88749.1.
      CR382361 Genomic DNA. No translation available.
      RefSeqiNP_001122195.1. NM_001128723.1.
      XP_009298009.1. XM_009299734.1.
      UniGeneiDr.148317.

      Genome annotation databases

      EnsembliENSDART00000076007; ENSDARP00000070486; ENSDARG00000074661.
      GeneIDi565271.
      KEGGidre:565271.

      Cross-referencesi

      Sequence databases

      Select the link destinations:
      EMBLi
      GenBanki
      DDBJi
      Links Updated
      EU652771 mRNA. Translation: ACD88749.1.
      CR382361 Genomic DNA. No translation available.
      RefSeqiNP_001122195.1. NM_001128723.1.
      XP_009298009.1. XM_009299734.1.
      UniGeneiDr.148317.

      3D structure databases

      ProteinModelPortaliB3G515.
      ModBaseiSearch...
      MobiDBiSearch...

      Protein-protein interaction databases

      STRINGi7955.ENSDARP00000070486.

      Protein family/group databases

      GPCRDBiSearch...

      Proteomic databases

      PaxDbiB3G515.

      Protocols and materials databases

      Structural Biology KnowledgebaseSearch...

      Genome annotation databases

      EnsembliENSDART00000076007; ENSDARP00000070486; ENSDARG00000074661.
      GeneIDi565271.
      KEGGidre:565271.

      Organism-specific databases

      CTDi2852.
      ZFINiZDB-GENE-090311-1. gper1.

      Phylogenomic databases

      eggNOGiENOG410IEGB. Eukaryota.
      ENOG4111653. LUCA.
      GeneTreeiENSGT00530000063910.
      HOGENOMiHOG000013114.
      HOVERGENiHBG005351.
      InParanoidiB3G515.
      KOiK04246.
      OMAiQHARLSC.
      OrthoDBiEOG7WX08J.
      PhylomeDBiB3G515.
      TreeFamiTF333506.

      Enzyme and pathway databases

      ReactomeiR-DRE-375276. Peptide ligand-binding receptors.
      R-DRE-418594. G alpha (i) signalling events.

      Miscellaneous databases

      PROiB3G515.

      Family and domain databases

      InterProiIPR000276. GPCR_Rhodpsn.
      IPR017452. GPCR_Rhodpsn_7TM.
      [Graphical view]
      PfamiPF00001. 7tm_1. 1 hit.
      [Graphical view]
      PRINTSiPR00237. GPCRRHODOPSN.
      PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
      PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
      [Graphical view]
      ProtoNetiSearch...

      Publicationsi

      « Hide 'large scale' publications
      1. "Identification of a membrane estrogen receptor in zebrafish with homology to mammalian GPER and its high expression in early germ cells of the testis."
        Liu X., Zhu P., Sham K.W., Yuen J.M., Xie C., Zhang Y., Liu Y., Li S., Huang X., Cheng C.H., Lin H.
        Biol. Reprod. 80:1253-1261(2009) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, ESTROGEN-BINDING, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
        Tissue: Testis.
      2. "The zebrafish reference genome sequence and its relationship to the human genome."
        Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
        , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
        Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
        Strain: Tuebingen.
      3. "Estrogen signaling characteristics of Atlantic croaker G protein-coupled receptor 30 (GPR30) and evidence it is involved in maintenance of oocyte meiotic arrest."
        Pang Y., Dong J., Thomas P.
        Endocrinology 149:3410-3426(2008) [PubMed] [Europe PMC] [Abstract]
        Cited for: FUNCTION.
      4. "G-protein-coupled estrogen receptor 1 is involved in brain development during zebrafish (Danio rerio) embryogenesis."
        Shi Y., Liu X., Zhu P., Li J., Sham K.W., Cheng S.H., Li S., Zhang Y., Cheng C.H., Lin H.
        Biochem. Biophys. Res. Commun. 435:21-27(2013) [PubMed] [Europe PMC] [Abstract]
        Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.

      Entry informationi

      Entry nameiGPER1_DANRE
      AccessioniPrimary (citable) accession number: B3G515
      Entry historyi
      Integrated into UniProtKB/Swiss-Prot: December 11, 2013
      Last sequence update: July 22, 2008
      Last modified: June 8, 2016
      This is version 59 of the entry and version 1 of the sequence. [Complete history]
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programChordata Protein Annotation Program

      Miscellaneousi

      Keywords - Technical termi

      Complete proteome, Reference proteome

      Documents

      1. SIMILARITY comments
        Index of protein domains and families

      Similar proteinsi

      Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
      100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
      90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
      50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.