B3EYN8 (G1PDH_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-1-phosphate dehydrogenase [NAD(P)+]
Short name=G1P dehydrogenase
Short name=G1PDH
EC=1.1.1.261

Alternative name(s):
Enantiomeric glycerophosphate synthase
sn-glycerol-1-phosphate dehydrogenase

Gene names

Name:	egsA
Synonyms:	araM

Organism

Geobacillus stearothermophilus (Bacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol-1-phosphate (G1P). The G1P thus generated is probably used for the synthesis of phosphoglycerolipids in Gram-positive bacterial species By similarity. HAMAP MF_00497_B
Catalytic activity	sn-glycerol-1-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H. HAMAP MF_00497_B
Cofactor	Binds 1 nickel ion per subunit By similarity. HAMAP MF_00497_B
Subunit structure	Homodimer By similarity. HAMAP MF_00497_B
Subcellular location	Cytoplasm Potential HAMAP MF_00497_B.
Sequence similarities	Belongs to the glycerol-1-phosphate dehydrogenase family.
Sequence caution	The sequence ACE73686.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding NAD NADP Nickel
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-1-phosphate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 404	404	Glycerol-1-phosphate dehydrogenase [NAD(P)+] HAMAP MF_00497_B		PRO_0000350640
Regions
	Nucleotide binding	118 – 122	5	NAD By similarity
	Nucleotide binding	140 – 143	4	NAD By similarity
Sites
	Metal binding	192	1	Nickel; catalytic By similarity
	Metal binding	272	1	Nickel; catalytic By similarity
	Metal binding	292	1	Nickel; catalytic By similarity
	Binding site	56	1	NAD By similarity
	Binding site	145	1	Substrate By similarity
	Binding site	149	1	NAD By similarity
	Binding site	192	1	Substrate By similarity
	Binding site	276	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3EYN8 [UniParc]. Last modified September 23, 2008. Version 2. Checksum: A0FB8FE86ED11027	FASTA	404	45,407
	10 20 30 40 50 60 MNQTLEEVVS LARQCGCGHR HYDIPIEQMV VGREAFARLV AYLRHKRYER VAIVADDHTF 70 80 90 100 110 120 AAVERSLCDQ LENGSIRYTV CLVQPDENGD VIADERSIVQ VLLETPDDVD VLIAVGAGTI 130 140 150 160 170 180 HDITRFSSYK MRIPFISVPT APSVDGFTSM GAPLIIRGVK KTIQAQAPIA VFAHTGVLCQ 190 200 210 220 230 240 SPKEMIAAGF GDMVAKYTSL ADWQFAHWMA DEPYCPFVHQ LTEQSLQTCV DHIDDIAAGG 250 260 270 280 290 300 EQGIRVLMDA LLQSGIAMLL MGQSYSASGA EHHLSHYWEM EFLRQKKRQV LHGAKVGVST 310 320 330 340 350 360 PIIIEHYQRV FWPLLNELEK RPKSMDEATW ERLKANTASI RELLESLPSP ERIRTMLAKV 370 380 390 400 GGAIAPEQLG IDPQLVERSL REAHRLRLNR FTMLYFLNEL IFVE

References

[1]	"Hemicellulose utilization cluster in Geobacillus stearothermophilus strain T-6." Shoham Y., Shulami S., Ben-David A., Langut Y. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: T-6 / NCIMB 40222.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ868502 Genomic DNA. Translation: ACE73686.1. Different initiation.
3D structure databases
ProteinModelPortal	B3EYN8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_00497_B. G1P_dehydrogenase_B. [Tree]
InterPro	IPR023003. G1P_dehydrogenase_bac. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G1PDH_GEOSE

Accession

Primary (citable) accession number: B3EYN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	September 23, 2008
Last modified:	June 28, 2011
This is version 20 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program