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B3EY95 (B3EY95_ACEAC) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 18. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein names

Succinyl-CoA:acetate coenzyme A transferase EMBL ACD85596.1
Gene names
Name:aarC EMBL ACD85596.1
OrganismAcetobacter aceti EMBL ACD85596.1
Taxonomic identifier435 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeAcetobacterAcetobacter subgen. Acetobacter

Protein attributes

Sequence length505 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region2 – 43Sulfate 1 binding
Region256 – 2583Sulfate 9 binding
Region408 – 4092Sulfate 10 binding

Sites

Binding site61Sulfate 2
Binding site111Sulfate 3
Binding site111Sulfate 4
Binding site361Citrate PDB 4EUD PDB 4EU7
Binding site711Acetate 1 PDB 4EU6 PDB 4FAC
Binding site711Citrate PDB 4EUD PDB 4EU7 PDB 4EU3
Binding site941Acetate 1 PDB 4EU6 PDB 4FAC
Binding site941Citrate PDB 4EUD PDB 4EU7 PDB 4EU3
Binding site1011Sulfate 5
Binding site1201Chloride 1 PDB 4EU7 PDB 4EU6 PDB 4EUB
Binding site1251Chloride 1 PDB 4EU5 PDB 4EU9 PDB 4EU8 PDB 4FAC PDB 4EUA PDB 4EU3
Binding site1411Sulfate 2; via amide nitrogen
Binding site1711Sulfate 6
Binding site1751Sulfate 7
Binding site1961Chloride 2; via amide nitrogen PDB 4EU6 PDB 4EU9 PDB 4EUA PDB 4EUC
Binding site1961Chloride 3; via amide nitrogen PDB 4FAC
Binding site2001Sulfate 8
Binding site2261Sulfate 6
Binding site2281Acetate 1 PDB 4EU6 PDB 4FAC
Binding site2281Citrate PDB 4EUD PDB 4EU7 PDB 4EU3
Binding site2941Acetate 2 (covalent) PDB 4EU6
Binding site2941Coenzyme A (covalent) PDB 4EU6 PDB 4EU9
Binding site3351Sulfate 7
Binding site3541Chloride 2 PDB 4EU6 PDB 4EU9 PDB 4EUA PDB 4EUC
Binding site3541Chloride 3 PDB 4EUD PDB 4EU5 PDB 4EU8 PDB 4FAC PDB 4EU3 PDB 4EUB
Binding site4081Sulfate 11; via amide nitrogen
Binding site4431Chloride 1; via amide nitrogen PDB 4EUD PDB 4EU5 PDB 4EU7 PDB 4EU6 PDB 4EU9 PDB 4EU8 PDB 4FAC PDB 4EUA PDB 4EU3 PDB 4EUB
Binding site4631Sulfate 12

Amino acid modifications

Modified residue2941N-actyl X PDB 4FAC

Sequences

Sequence LengthMass (Da)Tools
B3EY95 [UniParc].

Last modified July 22, 2008. Version 1.
Checksum: C02A8779588D2510

FASTA50554,826
        10         20         30         40         50         60 
MTERIRNVAL RSKVCPAETA SELIKHGDVV GTSGFTGAGY PKEVPKALAQ RMEAAHDRGE 

        70         80         90        100        110        120 
KYQISLITGA STGPQLDGEL AKANGVYFRS PFNTDATMRN RINAGETEYF DNHLGQVAGR 

       130        140        150        160        170        180 
AVQGNYGKFN IALVEATAIT EDGGIVPTSS VGNSQTFLNL AEKVIIEVNE WQNPMLEGIH 

       190        200        210        220        230        240 
DIWDGNVSGV PTRDIVPIVR ADQRVGGPVL RVNPDKIAAI VRTNDRDRNA PFAAPDETAK 

       250        260        270        280        290        300 
AIAGYLLDFF GHEVKQNRLP PSLLPLQSGV GNVANAVLEG LKEGPFENLV GYSEVIQDGM 

       310        320        330        340        350        360 
LAMLDSGRMR IASASSFSLS PEAAEEINNR MDFFRSKIIL RQQDVSNSPG IIRRLGCIAM 

       370        380        390        400        410        420 
NGMIEADIYG NVNSTRVMGS KMMNGIGGSG DFARSSYLSI FLSPSTAKGG KISAIVPMAA 

       430        440        450        460        470        480 
HVDHIMQDAQ IFVTEQGLAD LRGLSPVQRA REIISKCAHP DYRPMLQDYF DRALKNSFGK 

       490        500 
HTPHLLTEAL SWHQRFIDTG TMLPS 

« Hide

References

[1]"Structure of a NADH-insensitive hexameric citrate synthase that resists acid inactivation."
Francois J.A., Starks C.M., Sivanuntakorn S., Jiang H., Ransome A.E., Nam J.W., Constantine C.Z., Kappock T.J.
Biochemistry 45:13487-13499(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: 10-8 EMBL AGG68319.1 and 1023 EMBL ACD85596.1.
[2]"A specialized citric acid cycle requiring succinyl-coenzyme A (CoA):acetate CoA-transferase (AarC) confers acetic acid resistance on the acidophile Acetobacter aceti."
Mullins E.A., Francois J.A., Kappock T.J.
J. Bacteriol. 190:4933-4940(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: 10-8 EMBL AGG68319.1 and 1023 EMBL ACD85596.1.
[3]Kappock T.J.
Submitted (NOV-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: 1023 EMBL ACD85596.1.
[4]"Crystal structures of Acetobacter aceti succinyl-coenzyme A (CoA):acetate CoA-transferase reveal specificity determinants and illustrate the mechanism used by class I CoA-transferases."
Mullins E.A., Kappock T.J.
Biochemistry 51:8422-8434(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.48 ANGSTROMS) IN COMPLEX WITH ACETATE; CHLORIDE; CITRATE; COENZYME A AND SULFATE.
[5]"Succinyl-CoA:acetate CoA-transferase from Acetobacter aceti."
Mullins E.A., Kappock T.J.
Submitted (MAY-2012) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS) IN COMPLEX WITH ACETATE AND CHLORIDE, N-ACTYL X AT GLU-294.
[6]"Functional analysis of the acetic acid resistance (aar) gene cluster in Acetobacter aceti strain 1023."
Mullins E.A., Kappock T.J.
Acetic Acid Bacteria 2:e3-e3(2013)
Cited for: NUCLEOTIDE SEQUENCE.
Strain: 10-8 EMBL AGG68319.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ631551 Genomic DNA. Translation: ACD85596.1.
JX475924 Genomic DNA. Translation: AGG68319.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4EU3X-ray1.58A/B1-505[»]
4EU4X-ray2.80A/B1-505[»]
4EU5X-ray1.74A/B1-505[»]
4EU6X-ray1.99A/B1-505[»]
4EU7X-ray1.70A/B1-505[»]
4EU8X-ray1.81A/B1-505[»]
4EU9X-ray1.48A/B1-505[»]
4EUAX-ray2.40A/B1-505[»]
4EUBX-ray1.97A/B1-505[»]
4EUCX-ray2.64A/B1-505[»]
4EUDX-ray1.95A/B1-505[»]
4FACX-ray2.05A/B1-505[»]
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-17982.

Family and domain databases

InterProIPR026888. AcetylCoA_hyd_C.
IPR003702. ActCoA_hydro.
IPR017821. Succinate_CoA_transferase.
[Graphical view]
PfamPF13336. AcetylCoA_hyd_C. 1 hit.
PF02550. AcetylCoA_hydro. 1 hit.
[Graphical view]
TIGRFAMsTIGR03458. YgfH_subfam. 1 hit.
ProtoNetSearch...

Entry information

Entry nameB3EY95_ACEAC
AccessionPrimary (citable) accession number: B3EY95
Entry history
Integrated into UniProtKB/TrEMBL: July 22, 2008
Last sequence update: July 22, 2008
Last modified: June 11, 2014
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)