Phosphatidylinositol 4-kinase beta - Sorex araneus (Eurasian common shrew)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 4-kinase beta
Short name=PI4K-beta
Short name=PI4Kbeta
Short name=PtdIns 4-kinase beta
EC=2.7.1.67

Gene names

Name:	PI4KB
Synonyms:	PIK4CB

Organism

Sorex araneus (Eurasian common shrew) (European shrew)

Taxonomic identifier

42254 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Insectivora › Soricidae › Soricinae › Sorex

Protein attributes

Sequence length	801 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation By similarity. Involved in Golgi-to-plasma membrane trafficking By similarity.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 4-phosphate.
Enzyme regulation	Inhibited by wortmannin By similarity. Increased kinase activity upon interaction with NCS1/FREQ By similarity.
Subunit structure	Interacts with NCS1/FREQ in a calcium-independent manner By similarity. Interacts with CALN1/CABP8 and CALN2/CABP7; in a calcium-dependent manner By similarity. This binding competes with NCS1/FREQ binding By similarity. Interacts with ARF1 and ARF3 By similarity.
Subcellular location	Endomembrane system By similarity. Mitochondrion outer membrane; Peripheral membrane protein By similarity. Rough endoplasmic reticulum membrane; Peripheral membrane protein By similarity. Golgi apparatus By similarity. Note: Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-biphosphate (PIP2) on the Golgi complex By similarity.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily. Contains 1 PI3K/PI4K domain. Contains 1 PIK helical domain.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Golgi apparatus Membrane Mitochondrion Mitochondrion outer membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	phosphatidylinositol-mediated signaling Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell rough endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-phosphatidylinositol 4-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 801

801

Phosphatidylinositol 4-kinase beta

PRO_0000365168

Regions

Domain

29 – 242

214

PIK helical

Domain

559 – 765

207

PI3K/PI4K

Compositional bias

117 – 120

4

Poly-Arg

Amino acid modifications

Modified residue

258

1

Phosphoserine By similarity

Modified residue

263

1

Phosphothreonine By similarity

Modified residue

266

1

Phosphoserine By similarity

Modified residue

277

1

Phosphoserine By similarity

Modified residue

280

1

Phosphothreonine By similarity

Modified residue

294

1

Phosphoserine By similarity

Modified residue

413

1

Phosphoserine By similarity

Modified residue

496

1

Phosphoserine By similarity

Modified residue

502

1

Phosphothreonine By similarity

Modified residue

504

1

Phosphothreonine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

B3EX61 [UniParc].

Last modified July 22, 2008. Version 1.
Checksum: 63547909A887330A
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FASTA

801

89,991

        10         20         30         40         50         60 
MGDTAVEPAP LKPASEPAPG PPGNNGGSLL SVITEGVGEL SVIDPEVAQK ACQEVLEKVR 

        70         80         90        100        110        120 
LLHGAVAVSK RGTALELVNG DGVDTEIRCL DDPPAQIREE EDEMGATVTS GTAKGARRRR 

       130        140        150        160        170        180 
QNNSAKQSWL LRLFESKLFD ISMAISYLYN SKEPGVQAYI GNRLFCFRNE DVDFYLPQLL 

       190        200        210        220        230        240 
NMYIHMDEDV GDAIKPYIVH RCRQSINFSL QCALLLGAYS SDMHISTQRH SRGTKLRKLI 

       250        260        270        280        290        300 
LSDELKPAHR KRELPSLSPA LNTGLSPSKR THQRSKSDAT ASISLSSSLK RTASNPKVEN 

       310        320        330        340        350        360 
EDEPIRLAPE REFIKSLMAI GKRLATLPTK EQKTQRLISE LSLLNHKLPA RVWLPTAAFD 

       370        380        390        400        410        420 
HHVVRVPHTQ AVVLNSKDKA PYLIYVEVLE CENFDTTNVP ARIPENRIRS TRSVENLPEC 

       430        440        450        460        470        480 
GITHEQRAGS FSTVPNYDND DEAWSVDDIG ELQVELPEMH TNSCDNISQF SVDSITSQES 

       490        500        510        520        530        540 
KEPVFIAAGD IRRRLSEQLA HTPTAFRRDP EDPSAVALKE PWQEKVRRIR EGSPYGHLPN 

       550        560        570        580        590        600 
WRLLSVIVKC GDDLRQELLA FQVLKQLQSI WEQERVPLWI KPYKILVISA DSGMIEPVVN 

       610        620        630        640        650        660 
AVSIHQVKKQ SQLSLLDYFL QEHGSYTTEA FLSAQRNFVQ SCAGYCLVCY LLQVKDRHNG 

       670        680        690        700        710        720 
NILLDAEGHI IHIDFGFILS SSPRNLGFET SAFKLTTEFV DVMGGLDGDM FNYYKMLMLQ 

       730        740        750        760        770        780 
GLIAARKHMD KVVQIVEIMQ QGSQLPCFHG SSTIRNLKER FHMNMTEEQL QLLVEQMVDG 

       790        800 
SMRSITTKLY DGFQYLTNGI M

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References

[1]

"NISC comparative sequencing initiative."
Antonellis A., Ayele K., Benjamin B., Blakesley R.W., Boakye A., Bouffard G.G., Brinkley C., Brooks S., Chu G., Coleman H., Engle J., Gestole M., Greene A., Guan X., Gupta J., Haghighi P., Han J., Hansen N.

Green E.D.
Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	DP000772 Genomic DNA. Translation: ACE75819.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	maNOG12939.
HOVERGEN	HBG053597.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000403. PI3/4_kinase_cat. IPR018936. PI3/4_kinase_CS. IPR015433. PI_Kinase. [Graphical view]
Gene3D	G3DSA:1.10.1070.11. PI3/4_kinase_cat. 1 hit.
PANTHER	PTHR10048. PI_Kinase. 1 hit.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. [Graphical view]
SMART	SM00146. PI3Kc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. PS51545. PIK_HELICAL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PI4KB_SORAR

Accession

Primary (citable) accession number: B3EX61

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	July 22, 2008
Last modified:	October 19, 2011
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents