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Protein

Lectin alpha chain

Gene
N/A
Organism
Lablab purpureus (Hyacinth bean) (Dolichos lablab)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

D-galactose-binding lectin.1 Publication

pH dependencei

Optimum pH is 7.4. No activity between pH 4.0 and pH 6.0, 80% activity at pH 8.0 and 20% activity at pH 9.0.1 Publication

Temperature dependencei

Activity stable between 4 and 40 degrees Celsius, declines at higher temperatures and is lost at 90 degrees Celsius.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Fungicide

Keywords - Ligandi

Lectin

Names & Taxonomyi

Protein namesi
Recommended name:
Lectin alpha chain1 Publication
Alternative name(s):
DLL-II1 Publication
Cleaved into the following chain:
Lectin beta chain1 Publication
OrganismiLablab purpureus (Hyacinth bean) (Dolichos lablab)
Taxonomic identifieri35936 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeLablab

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004201301 – 281Lectin alpha chainAdd BLAST281
ChainiPRO_00004201311 – 263Lectin beta chain1 PublicationAdd BLAST263

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi35N-linked (GlcNAc...)Sequence analysis1
Glycosylationi82N-linked (GlcNAc...)Sequence analysis1
Glycosylationi140N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Glycosylated.1 Publication
The beta chain is produced by partial proteolytic processing of the alpha chain.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei263 – 264Cleavage1 Publication2

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiB3EWQ9.

Interactioni

Subunit structurei

Tetramer of 2 alpha and 2 beta chains.3 Publications

Structurei

Secondary structure

1281
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi25 – 33Combined sources9
Beta strandi39 – 41Combined sources3
Beta strandi51 – 53Combined sources3
Beta strandi68 – 75Combined sources8
Beta strandi82 – 84Combined sources3
Beta strandi87 – 97Combined sources11
Beta strandi108 – 116Combined sources9
Helixi125 – 127Combined sources3
Turni128 – 130Combined sources3
Beta strandi143 – 147Combined sources5
Beta strandi154 – 156Combined sources3
Beta strandi158 – 169Combined sources12
Beta strandi174 – 176Combined sources3
Beta strandi185 – 191Combined sources7
Turni193 – 195Combined sources3
Beta strandi197 – 203Combined sources7
Turni205 – 207Combined sources3
Beta strandi211 – 216Combined sources6
Turni220 – 222Combined sources3
Beta strandi225 – 235Combined sources11
Beta strandi237 – 241Combined sources5
Beta strandi246 – 256Combined sources11
Beta strandi258 – 260Combined sources3
Beta strandi264 – 267Combined sources4
Beta strandi270 – 272Combined sources3
Turni273 – 275Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UJOX-ray2.00A/B/C/D1-281[»]
3UJQX-ray2.06A/B/C/D1-281[»]
3UK9X-ray3.11A/B/C/D/E/F/G/H1-281[»]
3UL2X-ray2.50A/B/C/D1-281[»]
SMRiB3EWQ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the leguminous lectin family.Sequence analysis

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR016363. L-lectin.
IPR000985. Lectin_LegA_CS.
IPR019825. Lectin_legB_Mn/Ca_BS.
IPR001220. Legume_lectin_dom.
[Graphical view]
PfamiPF00139. Lectin_legB. 1 hit.
[Graphical view]
PIRSFiPIRSF002690. L-type_lectin_plant. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS00308. LECTIN_LEGUME_ALPHA. 1 hit.
PS00307. LECTIN_LEGUME_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B3EWQ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
NNLISFTMKR IVLFLILLTK AASANLISFT FKRFNETNLI LQRDATVSSG
60 70 80 90 100
KLRITKAAEN GVPTAGSLGR AFYSTPIQIW DNTTGTVAAW ATSFTFNLQA
110 120 130 140 150
PNAASPADGL AFALVPVGSQ PKDKGGFLGL FDSKNYASSN QTVAVEFDTF
160 170 180 190 200
YNGGWDPTER HIGIDVNSIK SIKTTSWDFA NGENAEVLIT YDSSTNLLVA
210 220 230 240 250
SLVHPSQKTS FIVSERVDLT SVLPEWVSVG FSATTGLSKG YVETNEVLSW
260 270 280
SFASKISINK EDEENKLLIS NLEGKAINNL A
Length:281
Mass (Da):30,422
Last modified:October 31, 2012 - v1
Checksum:iCC15AD7445BD170A
GO

Mass spectrometryi

Molecular mass is 30746 Da from positions 1 - 281. Determined by ESI. Alpha chain.1 Publication
Molecular mass is 28815 Da from positions 1 - 263. Determined by ESI. Beta chain.1 Publication

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3UJOX-ray2.00A/B/C/D1-281[»]
3UJQX-ray2.06A/B/C/D1-281[»]
3UK9X-ray3.11A/B/C/D/E/F/G/H1-281[»]
3UL2X-ray2.50A/B/C/D1-281[»]
SMRiB3EWQ9.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiB3EWQ9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR016363. L-lectin.
IPR000985. Lectin_LegA_CS.
IPR019825. Lectin_legB_Mn/Ca_BS.
IPR001220. Legume_lectin_dom.
[Graphical view]
PfamiPF00139. Lectin_legB. 1 hit.
[Graphical view]
PIRSFiPIRSF002690. L-type_lectin_plant. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS00308. LECTIN_LEGUME_ALPHA. 1 hit.
PS00307. LECTIN_LEGUME_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLECA2_LABPU
AccessioniPrimary (citable) accession number: B3EWQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2012
Last sequence update: October 31, 2012
Last modified: November 2, 2016
This is version 13 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.