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B3EWP2

- HYAL_CRODU

UniProt

B3EWP2 - HYAL_CRODU

Protein

Hyaluronidase CdtHya1

Gene
N/A
Organism
Crotalus durissus terrificus (South American rattlesnake)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Snake venom endo-hyaluronidase that degrades hyaluronan to smaller oligosaccharide fragments. In venom, it is not toxic by itself, but increases the diffusion of other venom proteins such as crotoxin (a neurotoxic and myotoxic PLA2) by degrading the extracellular matrix. In addition, it displays antiedematogenic activity, since it significantly diminishes the oedematogenic activity of crotoxin (probably by direct substrate hydrolysis, since hayluronan possesses strong water-binding capacity).2 Publications

    Catalytic activityi

    Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.By similarity

    pH dependencei

    Optimum pH is 5.5.2 Publications

    Temperature dependencei

    Optimum temperature is 37 degrees Celsius.2 Publications

    GO - Molecular functioni

    1. hyalurononglucosaminidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hyaluronidase CdtHya1 (EC:3.2.1.35By similarity)
    Short name:
    HyaBy similarity
    Alternative name(s):
    HyaluronoglucosaminidaseBy similarity
    Venom spreading factor
    OrganismiCrotalus durissus terrificus (South American rattlesnake)
    Taxonomic identifieri8732 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeCrotalus

    Subcellular locationi

    Secreted 2 Publications

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – ›44›44Hyaluronidase CdtHya1PRO_0000419017Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi24 ↔ ?By similarity

    Post-translational modificationi

    Contains disulfide bonds.
    Glycosylated.2 Publications

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.Curated

    Interactioni

    Subunit structurei

    Monomer.2 Publications

    Structurei

    3D structure databases

    ProteinModelPortaliB3EWP2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 56 family.Sequence Analysis

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR018155. Hyaluronidase.
    [Graphical view]
    PANTHERiPTHR11769. PTHR11769. 1 hit.
    PfamiPF01630. Glyco_hydro_56. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Fragment.

    B3EWP2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQAKAPMYPN EPFLVFWNAP TTQCRLRYKV DLDLNTFHIV TNAR         44
    Length:44
    Mass (Da):5,212
    Last modified:September 5, 2012 - v1
    Checksum:iDFD6932D5E3988B0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei44 – 4411 Publication

    Cross-referencesi

    3D structure databases

    ProteinModelPortali B3EWP2.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR017853. Glycoside_hydrolase_SF.
    IPR018155. Hyaluronidase.
    [Graphical view ]
    PANTHERi PTHR11769. PTHR11769. 1 hit.
    Pfami PF01630. Glyco_hydro_56. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Functional and structural characterization of hyaluronidase isolated from Crotalus durissus terrificus snake venom."
      Bordon K.C.F.
      Thesis (2012), University of Sao Paulo, Brazil
      Cited for: PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, GLYCOSYLATION, DISULFIDE BONDS.
      Tissue: Venom1 Publication.
    2. "Isolation, enzymatic characterization and antiedematogenic activity of the first reported rattlesnake hyaluronidase from Crotalus durissus terrificus venom."
      Bordon K.C.F., Perino M.G., Giglio J.R., Arantes E.C.
      Biochimie 94:2740-2748(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, GLYCOSYLATION, DISULFIDE BONDS.
      Tissue: Venom.

    Entry informationi

    Entry nameiHYAL_CRODU
    AccessioniPrimary (citable) accession number: B3EWP2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: September 5, 2012
    Last modified: October 1, 2014
    This is version 10 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3