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Protein

Hyaluronidase CdtHya1

Gene
N/A
Organism
Crotalus durissus terrificus (South American rattlesnake)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Snake venom endo-hyaluronidase that degrades hyaluronan to smaller oligosaccharide fragments. In venom, it is not toxic by itself, but increases the diffusion of other venom proteins such as crotoxin (a neurotoxic and myotoxic PLA2) by degrading the extracellular matrix. In addition, it displays antiedematogenic activity, since it significantly diminishes the oedematogenic activity of crotoxin (probably by direct substrate hydrolysis, since hayluronan possesses strong water-binding capacity).2 Publications

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.By similarity

pH dependencei

Optimum pH is 5.5.2 Publications

Temperature dependencei

Optimum temperature is 37 degrees Celsius.2 Publications

GO - Molecular functioni

  1. hyalurononglucosaminidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase CdtHya1 (EC:3.2.1.35By similarity)
Short name:
HyaBy similarity
Alternative name(s):
HyaluronoglucosaminidaseBy similarity
Venom spreading factor
OrganismiCrotalus durissus terrificus (South American rattlesnake)
Taxonomic identifieri8732 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeCrotalus

Subcellular locationi

Secreted 2 Publications

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – ›44›44Hyaluronidase CdtHya1PRO_0000419017Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi24 ↔ ?By similarity

Post-translational modificationi

Contains disulfide bonds.
Glycosylated.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed by the venom gland.Curated

Interactioni

Subunit structurei

Monomer.2 Publications

Structurei

3D structure databases

ProteinModelPortaliB3EWP2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.Sequence Analysis

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Fragment.

B3EWP2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40 
MQAKAPMYPN EPFLVFWNAP TTQCRLRYKV DLDLNTFHIV TNAR
Length:44
Mass (Da):5,212
Last modified:September 5, 2012 - v1
Checksum:iDFD6932D5E3988B0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei44 – 4411 Publication

Cross-referencesi

3D structure databases

ProteinModelPortaliB3EWP2.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Functional and structural characterization of hyaluronidase isolated from Crotalus durissus terrificus snake venom."
    Bordon K.C.F.
    Thesis (2011), University of Sao Paulo, Brazil
    Cited for: PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, GLYCOSYLATION, DISULFIDE BONDS.
    Tissue: Venom1 Publication.
  2. "Isolation, enzymatic characterization and antiedematogenic activity of the first reported rattlesnake hyaluronidase from Crotalus durissus terrificus venom."
    Bordon K.C.F., Perino M.G., Giglio J.R., Arantes E.C.
    Biochimie 94:2740-2748(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, GLYCOSYLATION, DISULFIDE BONDS.
    Tissue: Venom.

Entry informationi

Entry nameiHYAL_CRODU
AccessioniPrimary (citable) accession number: B3EWP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: September 5, 2012
Last modified: January 7, 2015
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.