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B3EWJ2

- LECA_DIOSC

UniProt

B3EWJ2 - LECA_DIOSC

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Protein

Lectin alpha chain

Gene
N/A
Organism
Dioclea sclerocarpa (Dioclea reflexa var. glabrescens)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

D-mannose/D-glucose-binding lectin. Has hemagglutinating activity towards rabbit erythrocytes.1 Publication

pH dependencei

Optimum pH is 6-8 for hemagglutinating activity. Activity is reduced to 50% at pH 8.5.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Manganese By similarityBy similarity
Metal bindingi10 – 101Calcium By similarityBy similarity
Metal bindingi10 – 101Manganese By similarityBy similarity
Metal bindingi12 – 121Calcium; via carbonyl oxygen By similarityBy similarity
Binding sitei12 – 121Carbohydrate By similarityBy similarity
Metal bindingi14 – 141Calcium By similarityBy similarity
Metal bindingi19 – 191Calcium By similarityBy similarity
Metal bindingi19 – 191Manganese By similarityBy similarity
Metal bindingi24 – 241Manganese By similarityBy similarity
Metal bindingi34 – 341Manganese By similarityBy similarity
Metal bindingi208 – 2081Calcium By similarityBy similarity
Binding sitei228 – 2281Carbohydrate; via amide nitrogen By similarityBy similarity

GO - Molecular functioni

  1. calcium ion binding Source: UniProtKB
  2. glucose binding Source: UniProtKB
  3. manganese ion binding Source: UniProtKB
  4. mannose binding Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Calcium, Lectin, Manganese, Mannose-binding, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lectin alpha chain
Alternative name(s):
DSL
Cleaved into the following 2 chains:
OrganismiDioclea sclerocarpa (Dioclea reflexa var. glabrescens)
Taxonomic identifieri1176036 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeDioclea

Subcellular locationi

Vacuolealeurone grain By similarity By similarity

GO - Cellular componenti

  1. aleurone grain Source: UniProtKB-SubCell
  2. vacuole Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 237237Lectin alpha chainPRO_0000417934Add
BLAST
Chaini1 – 118118Lectin beta chain1 PublicationPRO_0000417935Add
BLAST
Chaini119 – 237119Lectin gamma chain1 PublicationPRO_0000417936Add
BLAST

Post-translational modificationi

The beta and gamma chains are produced by partial proteolytic processing of the lectin alpha chain by an asparaginyl endopeptidase. Mixture of 60% alpha lectin and 40% of its beta and gamma proteolytic fragments.1 Publication

Interactioni

Subunit structurei

Homotetramer By similarity.By similarity

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni99 – 1002Carbohydrate binding By similarityBy similarity

Sequence similaritiesi

Belongs to the leguminous lectin family.

Family and domain databases

Gene3Di2.60.120.200. 2 hits.
InterProiIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000985. Lectin_LegA_CS.
IPR019825. Lectin_legB_Mn/Ca_BS.
IPR001220. Legume_lectin_dom.
[Graphical view]
PfamiPF00139. Lectin_legB. 2 hits.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS00308. LECTIN_LEGUME_ALPHA. 1 hit.
PS00307. LECTIN_LEGUME_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B3EWJ2-1 [UniParc]FASTAAdd to Basket

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ADTIVAVELD SYPNTDIGDP NYPHIGIDIK SIRSKSTARW NMQTGKVGTV    50
HISYNSVAKR LSAVVSYSGS SSTTVSYDVD LNNVLPEWVR VGLSATTGLY 100
KETNTILSWS FTSKLKTNSI ADENSLHFSF HKFSQNPKDL ILQGDAFTDS 150
DGNLQLTKVS SSGDPQGNSV GRALFYAPVH IWEKSAVVAS FDATFTFLIK 200
SPDREPADGI TFFIANTDTS IPSGSGGRLL GLFPDAN 237
Length:237
Mass (Da):25,607
Last modified:June 13, 2012 - v1
Checksum:iF33FD269ABF01D6A
GO

Mass spectrometryi

Molecular mass is 25606±2 Da from positions 1 - 237. Determined by ESI. Alpha chain.1 Publication
Molecular mass is 12873±2 Da from positions 1 - 118. Determined by ESI. Beta chain.1 Publication
Molecular mass is 12752±2 Da from positions 119 - 237. Determined by ESI. Gamma chain.1 Publication

Cross-referencesi

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.200. 2 hits.
InterProi IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000985. Lectin_LegA_CS.
IPR019825. Lectin_legB_Mn/Ca_BS.
IPR001220. Legume_lectin_dom.
[Graphical view ]
Pfami PF00139. Lectin_legB. 2 hits.
[Graphical view ]
SUPFAMi SSF49899. SSF49899. 1 hit.
PROSITEi PS00308. LECTIN_LEGUME_ALPHA. 1 hit.
PS00307. LECTIN_LEGUME_BETA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization and tandem mass spectrometry of the lectin extracted from the seeds of Dioclea sclerocarpa Ducke."
    Correia J.L., do Nascimento A.S., Cajazeiras J.B., Gondim A.C., Pereira R.I., de Sousa B.L., da Silva A.L., Garcia W., Teixeira E.H., do Nascimento K.S., da Rocha B.A., Nagano C.S., Sampaio A.H., Cavada B.S.
    Molecules 16:9077-9089(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, PTM, MASS SPECTROMETRY.
    Tissue: Seed.

Entry informationi

Entry nameiLECA_DIOSC
AccessioniPrimary (citable) accession number: B3EWJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: June 13, 2012
Last modified: February 19, 2014
This is version 9 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds one manganese (or another transition metal) ion and one calcium ion. The metal ions are essential for the saccharide-binding and cell-agglutinating activities.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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