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B3EWJ2 (LECA_DIOSC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 9. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lectin alpha chain
Alternative name(s):
DSL

Cleaved into the following 2 chains:

  1. Lectin beta chain
  2. Lectin gamma chain
OrganismDioclea sclerocarpa (Dioclea reflexa var. glabrescens)
Taxonomic identifier1176036 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeDioclea

Protein attributes

Sequence length237 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

D-mannose/D-glucose-binding lectin. Has hemagglutinating activity towards rabbit erythrocytes. Ref.1

Subunit structure

Homotetramer By similarity. UniProtKB P08902

Subcellular location

Vacuolealeurone grain By similarity UniProtKB P86624.

Post-translational modification

The beta and gamma chains are produced by partial proteolytic processing of the lectin alpha chain by an asparaginyl endopeptidase. Mixture of 60% alpha lectin and 40% of its beta and gamma proteolytic fragments. Ref.1

Miscellaneous

Binds one manganese (or another transition metal) ion and one calcium ion. The metal ions are essential for the saccharide-binding and cell-agglutinating activities.

Sequence similarities

Belongs to the leguminous lectin family.

Biophysicochemical properties

pH dependence:

Optimum pH is 6-8 for hemagglutinating activity. Activity is reduced to 50% at pH 8.5. Ref.1

Mass spectrometry

Molecular mass is 25606±2 Da from positions 1 - 237. Determined by ESI. Alpha chain. Ref.1

Molecular mass is 12873±2 Da from positions 1 - 118. Determined by ESI. Beta chain. Ref.1

Molecular mass is 12752±2 Da from positions 119 - 237. Determined by ESI. Gamma chain. Ref.1

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 237237Lectin alpha chain
PRO_0000417934
Chain1 – 118118Lectin beta chain Ref.1
PRO_0000417935
Chain119 – 237119Lectin gamma chain Ref.1
PRO_0000417936

Regions

Region99 – 1002Carbohydrate binding By similarity UniProtKB P08902

Sites

Metal binding81Manganese By similarity UniProtKB P08902
Metal binding101Calcium By similarity UniProtKB P08902
Metal binding101Manganese By similarity UniProtKB P08902
Metal binding121Calcium; via carbonyl oxygen By similarity UniProtKB P08902
Metal binding141Calcium By similarity UniProtKB P08902
Metal binding191Calcium By similarity UniProtKB P08902
Metal binding191Manganese By similarity UniProtKB P08902
Metal binding241Manganese By similarity UniProtKB P08902
Metal binding341Manganese By similarity UniProtKB P08902
Metal binding2081Calcium By similarity UniProtKB P08902
Binding site121Carbohydrate By similarity UniProtKB P08902
Binding site2281Carbohydrate; via amide nitrogen By similarity UniProtKB P08902

Sequences

Sequence LengthMass (Da)Tools
B3EWJ2 [UniParc].

Last modified June 13, 2012. Version 1.
Checksum: F33FD269ABF01D6A

FASTA23725,607
        10         20         30         40         50         60 
ADTIVAVELD SYPNTDIGDP NYPHIGIDIK SIRSKSTARW NMQTGKVGTV HISYNSVAKR 

        70         80         90        100        110        120 
LSAVVSYSGS SSTTVSYDVD LNNVLPEWVR VGLSATTGLY KETNTILSWS FTSKLKTNSI 

       130        140        150        160        170        180 
ADENSLHFSF HKFSQNPKDL ILQGDAFTDS DGNLQLTKVS SSGDPQGNSV GRALFYAPVH 

       190        200        210        220        230 
IWEKSAVVAS FDATFTFLIK SPDREPADGI TFFIANTDTS IPSGSGGRLL GLFPDAN 

« Hide

References

[1]"Molecular characterization and tandem mass spectrometry of the lectin extracted from the seeds of Dioclea sclerocarpa Ducke."
Correia J.L., do Nascimento A.S., Cajazeiras J.B., Gondim A.C., Pereira R.I., de Sousa B.L., da Silva A.L., Garcia W., Teixeira E.H., do Nascimento K.S., da Rocha B.A., Nagano C.S., Sampaio A.H., Cavada B.S.
Molecules 16:9077-9089(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, PTM, MASS SPECTROMETRY.
Tissue: Seed.

Cross-references

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.200. 2 hits.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000985. Lectin_LegA_CS.
IPR019825. Lectin_legB_Mn/Ca_BS.
IPR001220. Legume_lectin_dom.
[Graphical view]
PfamPF00139. Lectin_legB. 2 hits.
[Graphical view]
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS00308. LECTIN_LEGUME_ALPHA. 1 hit.
PS00307. LECTIN_LEGUME_BETA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLECA_DIOSC
AccessionPrimary (citable) accession number: B3EWJ2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: June 13, 2012
Last modified: February 19, 2014
This is version 9 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families