B3EWJ2 (LECA_DIOSC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Lectin alpha chain Alternative name(s): DSL Cleaved into the following 2 chains: Lectin beta chain Lectin gamma chain
Organism	Dioclea sclerocarpa (Dioclea reflexa var. glabrescens)
Taxonomic identifier	1176036 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Dioclea

Protein attributes

Sequence length	237 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	D-mannose/D-glucose-binding lectin. Has hemagglutinating activity towards rabbit erythrocytes. Ref.1
Subunit structure	Homotetramer By similarity. UniProtKB P08902
Subcellular location	Vacuole › aleurone grain By similarity UniProtKB P86624.
Post-translational modification	The beta and gamma chains are produced by partial proteolytic processing of the lectin alpha chain by an asparaginyl endopeptidase. Mixture of 60% alpha lectin and 40% of its beta and gamma proteolytic fragments. Ref.1
Miscellaneous	Binds one manganese (or other transition metal) ion and one calcium ion. The metal ions are essential for the saccharide-binding and cell-agglutinating activities. Ref.1 UniProtKB P08902
Sequence similarities	Belongs to the leguminous lectin family.
Biophysicochemical properties	pH dependence: Optimum pH is 6-8 for hemagglutinating activity. Activity is reduced to 50% at pH 8.5. Ref.1
Mass spectrometry	Molecular mass is 25606±2 Da from positions 1 - 237. Determined by ESI. Alpha chain. Ref.1 Molecular mass is 12873±2 Da from positions 1 - 118. Determined by ESI. Beta chain. Ref.1 Molecular mass is 12752±2 Da from positions 119 - 237. Determined by ESI. Gamma chain. Ref.1

Ontologies

Keywords
Cellular component	Vacuole
Ligand	Calcium Lectin Manganese Mannose-binding Metal-binding
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	aleurone grain Inferred from electronic annotation. Source: UniProtKB-SubCell vacuole Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	calcium ion binding Inferred from direct assay Ref.1. Source: UniProtKB glucose binding Inferred from direct assay Ref.1. Source: UniProtKB manganese ion binding Inferred from direct assay Ref.1. Source: UniProtKB mannose binding Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 237	237	Lectin alpha chain		PRO_0000417934
	Chain	1 – 118	118	Lectin beta chain Ref.1		PRO_0000417935
	Chain	119 – 237	119	Lectin gamma chain Ref.1		PRO_0000417936
Regions
	Region	99 – 100	2	Carbohydrate binding By similarity UniProtKB P08902
Sites
	Metal binding	8	1	Manganese By similarity UniProtKB P08902
	Metal binding	10	1	Calcium By similarity UniProtKB P08902
	Metal binding	10	1	Manganese By similarity UniProtKB P08902
	Metal binding	12	1	Calcium; via carbonyl oxygen By similarity UniProtKB P08902
	Metal binding	14	1	Calcium By similarity UniProtKB P08902
	Metal binding	19	1	Calcium By similarity UniProtKB P08902
	Metal binding	19	1	Manganese By similarity UniProtKB P08902
	Metal binding	24	1	Manganese By similarity UniProtKB P08902
	Metal binding	34	1	Manganese By similarity UniProtKB P08902
	Metal binding	208	1	Calcium By similarity UniProtKB P08902
	Binding site	12	1	Carbohydrate By similarity UniProtKB P08902
	Binding site	228	1	Carbohydrate; via amide nitrogen By similarity UniProtKB P08902

Sequences

	Sequence		Length	Mass (Da)
	B3EWJ2 [UniParc]. Last modified June 13, 2012. Version 1. Checksum: F33FD269ABF01D6A	FASTA	237	25,607
	10 20 30 40 50 60 ADTIVAVELD SYPNTDIGDP NYPHIGIDIK SIRSKSTARW NMQTGKVGTV HISYNSVAKR 70 80 90 100 110 120 LSAVVSYSGS SSTTVSYDVD LNNVLPEWVR VGLSATTGLY KETNTILSWS FTSKLKTNSI 130 140 150 160 170 180 ADENSLHFSF HKFSQNPKDL ILQGDAFTDS DGNLQLTKVS SSGDPQGNSV GRALFYAPVH 190 200 210 220 230 IWEKSAVVAS FDATFTFLIK SPDREPADGI TFFIANTDTS IPSGSGGRLL GLFPDAN

References

[1]

"Molecular characterization and tandem mass spectrometry of the lectin extracted from the seeds of Dioclea sclerocarpa Ducke." Correia J.L., do Nascimento A.S., Cajazeiras J.B., Gondim A.C., Pereira R.I., de Sousa B.L., da Silva A.L., Garcia W., Teixeira E.H., do Nascimento K.S., da Rocha B.A., Nagano C.S., Sampaio A.H., Cavada B.S. Molecules 16:9077-9089(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, PTM, MASS SPECTROMETRY. Tissue: Seed.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.60.120.200. 2 hits.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR000985. Lectin_LegA_CS. IPR019825. Lectin_legB_Mn/Ca_BS. IPR001220. Legume_lectin_dom. [Graphical view]
Pfam	PF00139. Lectin_legB. 2 hits. [Graphical view]
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS00308. LECTIN_LEGUME_ALPHA. 1 hit. PS00307. LECTIN_LEGUME_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LECA_DIOSC

Accession

Primary (citable) accession number: B3EWJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2012
Last sequence update:	June 13, 2012
Last modified:	March 6, 2013
This is version 6 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program