B3EWI9 (OXLA_BOTMT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: L-amino-acid oxidase Short name=Bm-LAO Short name=LAAO Short name=LAO EC=1.4.3.2
Organism	Bothropoides matogrossensis (Pitviper) (Bothrops neuwiedi matogrossensis)
Taxonomic identifier	1171125 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Viperidae › Crotalinae › Bothropoides

Protein attributes

Sequence length	31 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids, thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Exhibits diverse biological activities, such as hemorrhage, hemolysis, edema, antibacterial and antiparasitic activities, as well as regulation of platelet aggregation. Its effect on platelets is controversial, since it either induces aggregation or inhibits agonist-induced aggregation. These different effects are probably due to different experimental conditions By similarity. Inhibits growth of B.subtilis strain ATCC 6633 (MIC=32 µM), E.faecalis strain ATCC 12953 (MIC=32 µM), S.aureus strain ATCC 29213 (MIC=32 µM), S.pyogenes strain ATCC 19615 (MIC=8 µM), E.coli strain ATCC 8739 (MIC=4 µM), K.pneumoniae strain ATCC 13885 (MIC=2 µM), P.mirabilis strain ATCC 25933 (MIC=2 µM), P.aeruginosa strain ATCC 15442 (MIC=8 µM) and S.typhimurium strain ATCC 14028 (MIC=8 µM). Ref.1 UniProtKB Q6STF1
Catalytic activity	An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2. Ref.1
Cofactor	FAD By similarity. UniProtKB Q6STF1
Subunit structure	Homodimer; non-covalently linked By similarity. UniProtKB Q6STF1
Subcellular location	Secreted Ref.1.
Tissue specificity	Expressed by the venom gland. Ref.1
Post-translational modification	N-glycosylated By similarity. UniProtKB Q6STF1
Sequence similarities	Belongs to the flavin monoamine oxidase family. FIG1 subfamily.

Ontologies

Keywords
Biological process	Apoptosis Cytolysis Hemolysis
Cellular component	Secreted
Ligand	FAD Flavoprotein
Molecular function	Antibiotic Antimicrobial Hemostasis impairing toxin Oxidoreductase Toxin
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW hemolysis in other organism Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	L-amino-acid oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›31	›31	L-amino-acid oxidase		PRO_0000417918
Experimental info
	Non-adjacent residues	13 – 14	2
	Non-adjacent residues	21 – 22	2
	Non-terminal residue	1	1
	Non-terminal residue	31	1

Sequences

	Sequence		Length	Mass (Da)
	B3EWI9 [UniParc]. Last modified June 13, 2012. Version 1. Checksum: 778F60FA5F08EB99	FASTA	31	3,751
	10 20 30 IKFEPPLPPK KAHKKFWEDD GIYYPPNHNF P

References

[1]

"Evaluation of an antimicrobial L-amino acid oxidase and peptide derivatives from Bothropoides mattogrosensis pitviper venom." Okubo B.M., Silva O.N., Migliolo L., Gomes D.G., Porto W.F., Batista C.L., Ramos C.S., Holanda H.H., Dias S.C., Franco O.L., Moreno S.E. PLoS ONE 7:E33639-E33639(2012) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION. Tissue: Venom.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

OXLA_BOTMT

Accession

Primary (citable) accession number: B3EWI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2012
Last sequence update:	June 13, 2012
Last modified:	October 3, 2012
This is version 4 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Annotation program

Animal Toxin Annotation Program