B3EWH4 (NLTP_PEGHA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Non-specific lipid-transfer protein Short name=LTP Alternative name(s): Harmalin
Organism	Peganum harmala (Syrian rue) (Harmal peganum)
Taxonomic identifier	43879 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Sapindales › Nitrariaceae › Peganum

Protein attributes

Sequence length	20 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues. Inhibits cell proliferation of cervical carcinoma cell line HeLa (IC50=2.74 µM), gastric carcinoma cell line MGC-7 (IC50=3.13 µM), esophageal carcinoma cell line Eca-109 (IC50=0.7 µM) and melanoma B16 cells (IC50=1.47 µM). Has antifungal activity against P.digitatum (IC50=37.5 µM), A.alternata (IC50=1.5 µM), R.stolonifer (IC50=8.44 µM) and M.grisea (IC50=12.19 µM). Induces caspase-dependent apoptosis in cell line Eca-109. Demonstrates inhibitory effect on HIV-1 reverse transcriptase (IC50=1.26 µM). Ref.1 UniProtKB P82007
Subunit structure	Homodimer. Ref.1
Sequence similarities	Belongs to the plant LTP family.
Biophysicochemical properties	pH dependence: Stable between pH 4 and 10. Ref.1 Temperature dependence: Thermostable. Retains antifungal activity between 4 degrees Celsius and 60 degrees Celsius. Activity reduced after 30 min at 80 degrees Celsius. Ref.1

Ontologies

Keywords
Biological process	Transport
Ligand	Lipid-binding
Molecular function	Antimicrobial Fungicide
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	defense response to fungus Inferred from electronic annotation. Source: UniProtKB-KW killing of cells of other organism Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	lipid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›20	›20	Non-specific lipid-transfer protein		PRO_0000417400
Experimental info
	Non-terminal residue	20	1

Sequences

	Sequence		Length	Mass (Da)
	B3EWH4 [UniParc]. Last modified May 16, 2012. Version 1. Checksum: 994A3682BCA23404	FASTA	20	2,090
	10 20 ITCPQVTQSL APCVPYLISG

References

[1]

"Purification and characterization of a novel protein from Peganum harmala seeds with antifungal, antiproliferation and anti-HIV-1 reverse transcriptase activities." Ma X.J., Liu D.L., Tang H.S., Wang Y., Sun S.R. Submitted (MAR-2012) to UniProtKB Cited for: PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT. Tissue: Seed.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
PROSITE	PS00597. PLANT_LTP. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NLTP_PEGHA

Accession

Primary (citable) accession number: B3EWH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2012
Last sequence update:	May 16, 2012
Last modified:	March 6, 2013
This is version 4 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program