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Protein

Purotoxin-2

Gene
N/A
Organism
Geolycosa sp. (strain A267TDLS2-KZARNA) (Wolf spider) (Lycosa kazakhstanicus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Enhances the high-affinity desensitization of P2RX3 receptors. At 50 nM, decreases the IC50 for ambient ATP from 2.67 nM to 0.77 nM in human P2RX3. Does not affect mouse P2RX2 or P2RX2/P2RX3 receptors.1 Publication

GO - Molecular functioni

  • calcium channel inhibitor activity Source: UniProtKB
  • toxin activity Source: UniProtKB-KW

Keywordsi

Molecular functionCalcium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Purotoxin-21 Publication
Short name:
PT21 Publication
OrganismiGeolycosa sp. (strain A267TDLS2-KZARNA) (Wolf spider) (Lycosa kazakhstanicus)
Taxonomic identifieri420151 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeAraneomorphaeEntelegynaeLycosoideaLycosidaeGeolycosa

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PeptideiPRO_00004195281 – 64Purotoxin-21 PublicationAdd BLAST64

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi4 ↔ 19By similarity
Disulfide bondi11 ↔ 28By similarity
Disulfide bondi18 ↔ 44By similarity
Disulfide bondi30 ↔ 42By similarity
Modified residuei64Leucine amide1 Publication1

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.Curated

Structurei

Secondary structure

164
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 9Combined sources3
Helixi15 – 17Combined sources3
Beta strandi22 – 24Combined sources3
Beta strandi27 – 31Combined sources5
Turni36 – 38Combined sources3
Beta strandi41 – 45Combined sources5
Helixi48 – 63Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2MDNNMR-A1-64[»]
2MZFNMR-A1-64[»]
2MZGNMR-A1-64[»]
SMRiB3EWH0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.By similarity

Sequence similaritiesi

Belongs to the spider toxin CSTX superfamily.Sequence analysis

Keywords - Domaini

Knottin

Family and domain databases

InterProiView protein in InterPro
IPR019553. Spider_toxin_CSTX_knottin.
IPR011142. Spider_toxin_CSTX_Knottin_CS.
PfamiView protein in Pfam
PF10530. Toxin_35. 1 hit.
PROSITEiView protein in PROSITE
PS60029. SPIDER_CSTX. 1 hit.

Sequencei

Sequence statusi: Complete.

B3EWH0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
AKACTPLLHD CSHDRHSCCR GDMFKYVCDC FYPEGEDKTE VCSCQQPKSH
60
KIAEKIIDKA KTTL
Length:64
Mass (Da):7,264
Last modified:October 3, 2012 - v1
Checksum:iBB96626328CFCC59
GO

Mass spectrometryi

Molecular mass is 7255.5±0.5 Da from positions 1 - 64. Determined by MALDI. 1 Publication

Similar proteinsi

Entry informationi

Entry nameiTXPR2_GEOA2
AccessioniPrimary (citable) accession number: B3EWH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: October 3, 2012
Last modified: November 22, 2017
This is version 14 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families