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B3EWC6 (PPA_SCHCO) Reviewed, UniProtKB/Swiss-Prot

Last modified March 21, 2012. Version 2. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Acid phosphatase
EC=3.1.3.2
OrganismSchizophyllum commune (Split gill fungus)
Taxonomic identifier5334 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Agaricomycetes › Agaricomycetidae › Agaricales › Schizophyllaceae › Schizophyllum

Protein attributes

Sequence length10 AA.
Sequence statusFragment.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate. Ref.1

Enzyme regulation

Inhibited by metal ions like Fe2+, Fe3+, Cu2+, Hg2+ and Al3+. Ref.1

Biophysicochemical properties

Kinetic parameters:

KM=248 µM for para-nitrophenylphosphate (p-NPP) Ref.1

pH dependence:

Optimum pH is 4.6. Stable from pH 4 to 9. Ref.1

Temperature dependence:

Optimum temperature is 50 degrees Celsius. Stable from 20 to 50 degrees Celsius. Ref.1

Mass spectrometry

Molecular mass is 72500 Da from positions 1 - ?. Determined by ESI. Ref.1

Ontologies

Keywords
   Molecular functionHydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Molecular_functionacid phosphatase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – ›10›10Acid phosphatase
PRO_0000415411

Experimental info

Non-terminal residue101

Sequences

Sequence LengthMass (Da)Tools
B3EWC6 [UniParc].

Last modified January 25, 2012. Version 1.
Checksum: 2292ECA046DDC367

FASTA101,142
        10 
NAPWAQIDEV

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References

[1]"Purification and characterization of a novel acid phosphatase from the split gill mushroom Schizophyllum commune."
Zhang G.Q., Sun J., Wang H.X., Han C.H.
Submitted (DEC-2011) to UniProtKB
Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, MASS SPECTROMETRY.
Strain: 0805.
Tissue: Fruiting body.

Cross-references

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry namePPA_SCHCO
AccessionPrimary (citable) accession number: B3EWC6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 25, 2012
Last modified: March 21, 2012
This is version 2 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info