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B3EWC6 (PPA_SCHCO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 4. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Acid phosphatase

EC=3.1.3.2
OrganismSchizophyllum commune (Split gill fungus)
Taxonomic identifier5334 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesAgaricomycetidaeAgaricalesSchizophyllaceaeSchizophyllum

Protein attributes

Sequence length10 AA.
Sequence statusFragment.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate. Ref.1

Enzyme regulation

Activated slightly by Co2+, Ca2+ and Mg2+. Strongly inhibited by NaF, Fe3+ and Al3+, less so by tartrate, Cu2+ and Hg+. Not affected by EDTA, Fe2+ and Zn2+. Ref.1

Subunit structure

Monomer. Ref.1

Miscellaneous

Has no activity towards sodium phytate, ATP, ADP, AMP, glucose-6-phosphate, fructose-6-phosphate and beta-glycerophosphate. Ref.1

Biophysicochemical properties

Kinetic parameters:

KM=248 µM for para-nitrophenylphosphate (p-NPP) Ref.1

Vmax=9 nM/min/mg enzyme Ref.1

pH dependence:

Optimum pH is 4.6. Activity decreases sharply with decreases and increases in pH and is absent at pH 8.0. When pre-incubated at different pH levels, activity is stable between pH 4 and 9. Ref.1

Temperature dependence:

Optimum temperature is 50 degrees Celsius. Activity decreases sharply with decreases and increases in temperature. When pre-incubated at different temperatures, activity is stable between 20 and 50 degrees Celsius. Ref.1

Ontologies

Keywords
   Molecular functionHydrolase
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processdephosphorylation

Inferred from direct assay Ref.1. Source: UniProtKB

   Molecular_functionacid phosphatase activity

Inferred from direct assay Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – ›10›10Acid phosphatase
PRO_0000415411

Experimental info

Non-terminal residue101

Sequences

Sequence LengthMass (Da)Tools
B3EWC6 [UniParc].

Last modified January 25, 2012. Version 1.
Checksum: 2292ECA046DDC367

FASTA101,142
        10 
NAPWAQIDEV 

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References

[1]"Purification and characterization of a novel acid phosphatase from the split gill mushroom Schizophyllum commune."
Zhang G.Q., Chen Q.J., Sun J., Wang H.X., Han C.H.
J. Basic Microbiol. 53:1-8(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
Strain: 0805.
Tissue: Fruiting body.

Cross-references

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry namePPA_SCHCO
AccessionPrimary (citable) accession number: B3EWC6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 25, 2012
Last modified: April 16, 2014
This is version 4 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program