B3EWC6 (PPA_SCHCO) Reviewed, UniProtKB/Swiss-Prot
Last modified April 16, 2014. Version 4. History...
Names and origin
|Sequence length||10 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
A phosphate monoester + H2O = an alcohol + phosphate. Ref.1
Activated slightly by Co2+, Ca2+ and Mg2+. Strongly inhibited by NaF, Fe3+ and Al3+, less so by tartrate, Cu2+ and Hg+. Not affected by EDTA, Fe2+ and Zn2+. Ref.1
Has no activity towards sodium phytate, ATP, ADP, AMP, glucose-6-phosphate, fructose-6-phosphate and beta-glycerophosphate. Ref.1
KM=248 µM for para-nitrophenylphosphate (p-NPP) Ref.1
Vmax=9 nM/min/mg enzyme Ref.1
Optimum pH is 4.6. Activity decreases sharply with decreases and increases in pH and is absent at pH 8.0. When pre-incubated at different pH levels, activity is stable between pH 4 and 9. Ref.1
Optimum temperature is 50 degrees Celsius. Activity decreases sharply with decreases and increases in temperature. When pre-incubated at different temperatures, activity is stable between 20 and 50 degrees Celsius. Ref.1
|Technical term||Direct protein sequencing|
|Gene Ontology (GO)|
|Molecular_function||acid phosphatase activity|
|Complete GO annotation...|
Sequence annotation (Features)
|||"Purification and characterization of a novel acid phosphatase from the split gill mushroom Schizophyllum commune."|
Zhang G.Q., Chen Q.J., Sun J., Wang H.X., Han C.H.
J. Basic Microbiol. 53:1-8(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
Tissue: Fruiting body.
|Accession||Primary (citable) accession number: B3EWC6|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Fungal Protein Annotation Program|