B3EUH5 (SYD_AMOA5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	Aasi_0148

Organism

Amoebophilus asiaticus (strain 5a2) [Complete proteome] [HAMAP]

Taxonomic identifier

452471 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Candidatus Amoebophilus

Protein attributes

Sequence length	582 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 582	582	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_1000090956

Sequences

	Sequence		Length	Mass (Da)
	B3EUH5 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: EA9A522F4C41EFB1	FASTA	582	66,419
	10 20 30 40 50 60 MLRTHTCGEL RLQHIGISVT LCGWVQKIRN KGSLVWIDLR DRYGITQLIL EEHITAPEIL 70 80 90 100 110 120 SQVQHIGREY VIQATGSVIE RSAKNPSMPT GDIEIEVKSL TILNTAKTPP FLIEEQTDGG 130 140 150 160 170 180 EELRMQYRYL DLRRPPLQKN LLLRQLVAQH ARAYLEQHHF VDVETPLLIK STPGGARDFV 190 200 210 220 230 240 VPSRIHPQQF YALPQSPQIF KQLLMVAGLD RYYQIAKCFR DEDFRADRQP EFTQIDCELS 250 260 270 280 290 300 FVTQADILHI FENFTKYIFE ATIQVRLDKF PCITYAEAMQ KYGTDKPDIR FGMRLIELTE 310 320 330 340 350 360 LVKNSEFPLF KQAKLIAGIC VKGCADYTRK QLDDLTEYIK KLNLVTSGLV YVKYLADGSF 370 380 390 400 410 420 NSPVSKFYDV EQLTLWAKQM HAVPGDLLLI LAGEIEATQI ALGSLRLKLR DELHLVSKDK 430 440 450 460 470 480 FAPLWVVDFP LLEWNEESQR YVSRHHPFTS PKQEDIELLS TKPETVRANA YDLVINGMEI 490 500 510 520 530 540 GGGSIRIHDR ALQEQIFNVL GFSEEEARQQ FGFLTDAFEY GAPPHGGIAF GFDRLCAIIG 550 560 570 580 REDSIRPFIA FPKNNAGRDV MMKAPSTITE QQISELGIIL SK

References

[1]

"Complete sequence of Candidatus Amoebophilus asiaticus 5a2." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Horn M., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 5a2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001102 Genomic DNA. Translation: ACE05594.1.
RefSeq	YP_001957323.1. NC_010830.1.
3D structure databases
ProteinModelPortal	B3EUH5.
ModBase	Search...
Protein-protein interaction databases
STRING	B3EUH5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6376390.
GenomeReviews	Gene locus Aasi_0148 in contig CP001102_GR.
KEGG	aas:Aasi_0148.
PATRIC	21265613. VBICanAmo76781_0165.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG396032.
OMA	AFPKTQQ.
ProtClustDB	PRK00476.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_AMOA5

Accession

Primary (citable) accession number: B3EUH5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program