B3EUF3 (EFG_AMOA5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor G
Short name=EF-G

Gene names

Name:	fusA
Ordered Locus Names:	Aasi_0125

Organism

Amoebophilus asiaticus (strain 5a2) [Complete proteome] [HAMAP]

Taxonomic identifier

452471 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Candidatus Amoebophilus

Protein attributes

Sequence length	709 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP MF_00054_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00054_B.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 709	709	Elongation factor G HAMAP MF_00054_B		PRO_1000091690
Regions
	Nucleotide binding	15 – 22	8	GTP By similarity
	Nucleotide binding	92 – 96	5	GTP By similarity
	Nucleotide binding	146 – 149	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3EUF3 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 685FAACF006A7913	FASTA	709	78,522
	10 20 30 40 50 60 MAKDLKFLRN IGIMAHIDAG KTTTTERILY YTGLTHKIGE VHEGGAVMDW MEQEQERGIT 70 80 90 100 110 120 ITAAATTTFW KYPTVQGQVT KDTQQYSINI IDTPGHVDFT VEVERSLRVL DGAVALFCAV 130 140 150 160 170 180 SGVEPQSETV WRQADKYRVP RICFVNKMDR AGADFFNAVS EIKSKLGANP IPLQIPIGSE 190 200 210 220 230 240 DTFKGVVDLI RNQAIIWNDE DLGMTYQVVP IPEDLKETVE EYRQKLMESV AEYDEVLLEK 250 260 270 280 290 300 YFNDPNTITT EEIMVAIRKA VIDMSISPVL CGASFKNKGV QALLDAVCTY LPSPLDLPPV 310 320 330 340 350 360 QGTNPDTEEK QIRKPDVNEP FAALAFKIAT DPFVGRLAFM RVYSGKLDGS SYVYNNRTGK 370 380 390 400 410 420 KERISRLMQM HANKQNPIDS IEAGDICAAV GFKDIRTGDT LTDEKNKIIL ESIVFPEPVI 430 440 450 460 470 480 GYSIEPKKQA DVDKLSMAIS KLVEEDPTLH VETNEETGQT VMKGMGELHL EIIIDRLRRE 490 500 510 520 530 540 FKLEINQGAP QVAYKEALTS TIEHKEVYKK QTGGRGKFAD IVFELGPRED GQMGLQFTNE 550 560 570 580 590 600 IVGGAIPREF IPAIQKGFAA AMANGPLASY PVESMKVKLI HGSFHEVDSD SLSFELAARI 610 620 630 640 650 660 GFKEAAKKAS PVIMEPIMAV EVTTPDEFTG PVTGDLNKRR GIMRGMDAKG NTQVVKADVP 670 680 690 700 LSELFGYVTD LRTITSGRAA ATLTFSRYEQ VPKQLADSIV EKVKGTANK

References

[1]

"Complete sequence of Candidatus Amoebophilus asiaticus 5a2." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Horn M., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 5a2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001102 Genomic DNA. Translation: ACE05572.1.
RefSeq	YP_001957301.1. NC_010830.1.
3D structure databases
ProteinModelPortal	B3EUF3.
ModBase	Search...
Protein-protein interaction databases
STRING	B3EUF3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6376591.
GenomeReviews	Gene locus Aasi_0125 in contig CP001102_GR.
KEGG	aas:Aasi_0125.
PATRIC	21265565. VBICanAmo76781_0141.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG737692.
OMA	CNEWREK.
ProtClustDB	PRK12739.
Family and domain databases
HAMAP	MF_00054_B. EF-G_EF-2_B. [Tree]
InterPro	IPR009022. Elongation_fac_G/III/V. IPR000795. ProtSyn_GTP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP-bd_dom. IPR004540. Transl_elong_EFG/EF2. IPR000640. Transl_elong_EFG/EF2_C. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.
KO	K02355.
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00484. EF-G. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFG_AMOA5

Accession

Primary (citable) accession number: B3EUF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program