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Protein

Elongation factor Tu

Gene

tuf

Organism
Amoebophilus asiaticus (strain 5a2)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 268GTPUniRule annotation
Nucleotide bindingi81 – 855GTPUniRule annotation
Nucleotide bindingi136 – 1394GTPUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAASI452471:GKEN-1542-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor TuUniRule annotation
Short name:
EF-TuUniRule annotation
Gene namesi
Name:tufUniRule annotation
Ordered Locus Names:Aasi_1403
OrganismiAmoebophilus asiaticus (strain 5a2)
Taxonomic identifieri452471 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesCytophagiaCytophagalesAmoebophilaceaeCandidatus Amoebophilus
ProteomesiUP000001227 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 395395Elongation factor TuPRO_1000095047Add
BLAST

Proteomic databases

PRIDEiB3ETZ7.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi452471.Aasi_1403.

Structurei

3D structure databases

ProteinModelPortaliB3ETZ7.
SMRiB3ETZ7. Positions 2-395.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 204195tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni19 – 268G1By similarity
Regioni60 – 645G2By similarity
Regioni81 – 844G3By similarity
Regioni136 – 1394G4By similarity
Regioni174 – 1763G5By similarity

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0050.
HOGENOMiHOG000229290.
KOiK02358.
OMAiDYGEIDK.
OrthoDBiEOG6R5C6X.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B3ETZ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKETFDRSK PHVNIGTIGH VDHGKTTLTA AITKVLSKRG LAQVRDFGSI
60 70 80 90 100
DNAPEERERG ITINTSHVEY ETSKRHYAHV DCPGHADYVK NMITGAAQMD
110 120 130 140 150
GAILVVAATD GPMPQTREHI LLASQVGVPN LVVFLNKVDA VDDPELVDLV
160 170 180 190 200
EEEVRDLLKA YKFDGDNIPV IRGSALGALN GEPEWEAKVE ELMDNVDEYI
210 220 230 240 250
PLPQRLIDRD FLMPVEDTMS ITGRGTVATG RIERGVINVG DPVQIIGMGA
260 270 280 290 300
QNLNSTVTGV EMFRKLLDRG EAGDNVGLLL RGIDKEKIHR GMVICKPKSV
310 320 330 340 350
TPHRKFKAEV YVLSKEEGGR HTPFFNKYRP QFYFRTTDVT GEVKLPAGVE
360 370 380 390
MVMPGDNIAL EVELINEIAM EKGLRFAIRE GGRTVGAGQI IEILD
Length:395
Mass (Da):43,514
Last modified:July 22, 2008 - v1
Checksum:i88A6ACCC64AAC333
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001102 Genomic DNA. Translation: ACE06699.1.
RefSeqiWP_012473441.1. NC_010830.1.
YP_001958428.1. NC_010830.1.

Genome annotation databases

EnsemblBacteriaiACE06699; ACE06699; Aasi_1403.
KEGGiaas:Aasi_1403.
PATRICi21268617. VBICanAmo76781_1630.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001102 Genomic DNA. Translation: ACE06699.1.
RefSeqiWP_012473441.1. NC_010830.1.
YP_001958428.1. NC_010830.1.

3D structure databases

ProteinModelPortaliB3ETZ7.
SMRiB3ETZ7. Positions 2-395.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi452471.Aasi_1403.

Proteomic databases

PRIDEiB3ETZ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACE06699; ACE06699; Aasi_1403.
KEGGiaas:Aasi_1403.
PATRICi21268617. VBICanAmo76781_1630.

Phylogenomic databases

eggNOGiCOG0050.
HOGENOMiHOG000229290.
KOiK02358.
OMAiDYGEIDK.
OrthoDBiEOG6R5C6X.

Enzyme and pathway databases

BioCyciAASI452471:GKEN-1542-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00118_B. EF_Tu_B.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
IPR004161. Transl_elong_EFTu/EF1A_2.
IPR004541. Transl_elong_EFTu/EF1A_bac/org.
IPR004160. Transl_elong_EFTu/EF1A_C.
[Graphical view]
PfamiPF03144. GTP_EFTU_D2. 1 hit.
PF03143. GTP_EFTU_D3. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00485. EF-Tu. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of the amoeba symbiont 'Candidatus Amoebophilus asiaticus' reveals common mechanisms for host cell interaction among amoeba-associated bacteria."
    Schmitz-Esser S., Tischler P., Arnold R., Montanaro J., Wagner M., Rattei T., Horn M.
    J. Bacteriol. 192:1045-1057(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 5a2.

Entry informationi

Entry nameiEFTU_AMOA5
AccessioniPrimary (citable) accession number: B3ETZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 22, 2008
Last modified: June 24, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.