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B3ETW5 (SYL_AMOA5) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Aasi_1363
OrganismAmoebophilus asiaticus (strain 5a2) [Complete proteome] [HAMAP]
Taxonomic identifier452471 [NCBI]
Taxonomic lineageBacteriaBacteroidetesCytophagiaCytophagalesAmoebophilaceaeCandidatus Amoebophilus

Protein attributes

Sequence length923 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 923923Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091287

Regions

Motif41 – 5212"HIGH" region HAMAP-Rule MF_00049
Motif698 – 7025"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site7011ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B3ETW5 [UniParc].

Last modified July 22, 2008. Version 1.
Checksum: AAB6AE51D668398C

FASTA923105,607
        10         20         30         40         50         60 
MSHYDFKAIE QKWQQYWKNN QIFRTTIEPS KPKYYILDMF PYPSGEGLHV GHPLGYIASD 

        70         80         90        100        110        120 
IVARYKRSKG YQVLHPMGFD AFGLPAEQFA IQTGQHPAIT TAKNIGRYKQ QLCQLGLSYD 

       130        140        150        160        170        180 
WDRCISTCEP AYYKWTQWIF IQLFNSWYDI SLQKARPIDE LITLFDQQGN QQVQASCDKE 

       190        200        210        220        230        240 
VSLFTAKEWQ AMDEESKQQH LLAYRLAFLE DTTVNWCPEL GTVLANEEVK DGLSERGGYP 

       250        260        270        280        290        300 
VIRKQMKQWS LRITAYTDRL LAGLEHLKWP LSTKEMQRNW IGRSIGAELN FTVIANGQEH 

       310        320        330        340        350        360 
TIPVFTTRPD TLFGVTYLAL SPEHPLAKLI STGTQQAAID TYITQATNRS ERDRLADVNH 

       370        380        390        400        410        420 
VTGMFTGAYA IHPFTKQPLP IWIADYVLAG YGTGAVMGVP AHDSRDYAFA QHFQLPIIQV 

       430        440        450        460        470        480 
VAGGDTAQSA YEAREGSLFN SQFLNGLSIQ EATKQAIQKL ESLGIGKQKT TYRLRNAIFS 

       490        500        510        520        530        540 
RQRYWGEPIP IYYKNNIPYP IPAEELPLEL PSLASFKPTP TGEPPLGHAP NWKTKEGYPI 

       550        560        570        580        590        600 
ELSTMPGWAG SSWYFFRYMD PNNEASFVGS TAQNYWQAVD LYLGGAEHAT GHLLYARFWT 

       610        620        630        640        650        660 
QFLYDLGYVN IEEPFQELIH QGMIQGKSSF VYRIKGTNQF VSYNLRHAYE TTAMHVDIHL 

       670        680        690        700        710        720 
VKNNILDLER FKNWRPDLQT ATFVLENGQY ICGSEVEKMS KSKYNTVNPD TVVEQYGADT 

       730        740        750        760        770        780 
LRLYTMFLGP IEQAKPWDMH GIEGVFRFLV KVWRLFYLEK GAIITNEVPT KEVQKAIHKA 

       790        800        810        820        830        840 
IKKVEEDIKR YAFNTAVSNL MICVNELTAL KCNNRAALTN LVLILAPFAP HLAEELWEIL 

       850        860        870        880        890        900 
GHQHSIAQAP FPTYEEIYLQ EETYEYPIAI NGKVRAKINF PVDMPQGQIE EQVLTHESIQ 

       910        920 
KWIQGQQIKR VIVISSKMVN IVI 

« Hide

References

[1]"Complete sequence of Candidatus Amoebophilus asiaticus 5a2."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Horn M., Richardson P.
Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 5a2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001102 Genomic DNA. Translation: ACE06667.1.
RefSeqYP_001958396.1. NC_010830.1.

3D structure databases

ProteinModelPortalB3ETW5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING452471.Aasi_1363.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACE06667; ACE06667; Aasi_1363.
GeneID6377662.
KEGGaas:Aasi_1363.
PATRIC21268499. VBICanAmo76781_1573.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAMAFAGPP.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycAASI452471:GKEN-1493-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_AMOA5
AccessionPrimary (citable) accession number: B3ETW5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 22, 2008
Last modified: July 9, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries