B3ERQ7 (SYE_AMOA5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	Aasi_0504

Organism

Amoebophilus asiaticus (strain 5a2) [Complete proteome] [HAMAP]

Taxonomic identifier

452471 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Candidatus Amoebophilus

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 512	512	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000367607
Regions
	Motif	11 – 21	11	"HIGH" region HAMAP MF_00022_B
	Motif	263 – 267	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	266	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3ERQ7 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: B2CFC352FC53C85D	FASTA	512	58,508
	10 20 30 40 50 60 MEKPIRVRFA PSPTGALHIG GVRTALYNYL LARKHQGKFI LRIEDTDQNR FVPGAEQYII 70 80 90 100 110 120 DTLQWLGIDP DEGIQQGGPF APYRQSDRKD MYRKYADQLV QAGKAYYAFD TPEELEAMRE 130 140 150 160 170 180 RLQAAKVASP QYNAISREWM KNSLTLPQEE VTARINAGEP YVIRFKMPHK EIVRFYDQVR 190 200 210 220 230 240 GWVKVETSTL DDKVLLKSDG MATYHLANVV DDYLMQISHV IRGEEWLPSA PLHILLYQAF 250 260 270 280 290 300 GWEQTMPQFV HLPILLKPEG HGKLSKRDAD KHGFPIFPIA WQDPATGNHI EGFREKGYLP 310 320 330 340 350 360 EALINFLALL GWSPGGDQEL FTKEALVEAF SLERIGKSGV KFDIQKANWF NQQYLRNKTE 370 380 390 400 410 420 KELSVYLTSE LDKREIAYTT EQAEQICALV KERAIFPQDF WEQGQVFFQA PTTYDAQAIQ 430 440 450 460 470 480 KRWTSQAHET LAGFVDILPT ISPFNAASIK ESLADFLKER SIKINEMMPV IRIALMGTTA 490 500 510 GPDLMQSIEI IGQKETIRRL RTALRIIVPV GS

References

[1]

"Complete sequence of Candidatus Amoebophilus asiaticus 5a2." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Horn M., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 5a2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001102 Genomic DNA. Translation: ACE05909.1.
RefSeq	YP_001957638.1. NC_010830.1.
3D structure databases
ProteinModelPortal	B3ERQ7.
ModBase	Search...
Protein-protein interaction databases
STRING	B3ERQ7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6376537.
GenomeReviews	Gene locus Aasi_0504 in contig CP001102_GR.
KEGG	aas:Aasi_0504.
PATRIC	21266465. VBICanAmo76781_0583.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	IEWFNLD.
ProtClustDB	PRK01406.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_AMOA5

Accession

Primary (citable) accession number: B3ERQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program