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B3ERQ7

- SYE_AMOA5

UniProt

B3ERQ7 - SYE_AMOA5

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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Amoebophilus asiaticus (strain 5a2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei266 – 2661ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciAASI452471:GKEN-555-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Aasi_0504
OrganismiAmoebophilus asiaticus (strain 5a2)
Taxonomic identifieri452471 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesCytophagiaCytophagalesAmoebophilaceaeCandidatus Amoebophilus
ProteomesiUP000001227: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 512512Glutamate--tRNA ligasePRO_0000367607Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi452471.Aasi_0504.

Structurei

3D structure databases

ProteinModelPortaliB3ERQ7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi11 – 2111"HIGH" regionAdd
BLAST
Motifi263 – 2675"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiVTGQTHG.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B3ERQ7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEKPIRVRFA PSPTGALHIG GVRTALYNYL LARKHQGKFI LRIEDTDQNR
60 70 80 90 100
FVPGAEQYII DTLQWLGIDP DEGIQQGGPF APYRQSDRKD MYRKYADQLV
110 120 130 140 150
QAGKAYYAFD TPEELEAMRE RLQAAKVASP QYNAISREWM KNSLTLPQEE
160 170 180 190 200
VTARINAGEP YVIRFKMPHK EIVRFYDQVR GWVKVETSTL DDKVLLKSDG
210 220 230 240 250
MATYHLANVV DDYLMQISHV IRGEEWLPSA PLHILLYQAF GWEQTMPQFV
260 270 280 290 300
HLPILLKPEG HGKLSKRDAD KHGFPIFPIA WQDPATGNHI EGFREKGYLP
310 320 330 340 350
EALINFLALL GWSPGGDQEL FTKEALVEAF SLERIGKSGV KFDIQKANWF
360 370 380 390 400
NQQYLRNKTE KELSVYLTSE LDKREIAYTT EQAEQICALV KERAIFPQDF
410 420 430 440 450
WEQGQVFFQA PTTYDAQAIQ KRWTSQAHET LAGFVDILPT ISPFNAASIK
460 470 480 490 500
ESLADFLKER SIKINEMMPV IRIALMGTTA GPDLMQSIEI IGQKETIRRL
510
RTALRIIVPV GS
Length:512
Mass (Da):58,508
Last modified:July 22, 2008 - v1
Checksum:iB2CFC352FC53C85D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001102 Genomic DNA. Translation: ACE05909.1.
RefSeqiWP_012472674.1. NC_010830.1.
YP_001957638.1. NC_010830.1.

Genome annotation databases

EnsemblBacteriaiACE05909; ACE05909; Aasi_0504.
GeneIDi6376537.
KEGGiaas:Aasi_0504.
PATRICi21266465. VBICanAmo76781_0583.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001102 Genomic DNA. Translation: ACE05909.1 .
RefSeqi WP_012472674.1. NC_010830.1.
YP_001957638.1. NC_010830.1.

3D structure databases

ProteinModelPortali B3ERQ7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 452471.Aasi_0504.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACE05909 ; ACE05909 ; Aasi_0504 .
GeneIDi 6376537.
KEGGi aas:Aasi_0504.
PATRICi 21266465. VBICanAmo76781_0583.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252720.
KOi K01885.
OMAi VTGQTHG.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci AASI452471:GKEN-555-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of the amoeba symbiont 'Candidatus Amoebophilus asiaticus' reveals common mechanisms for host cell interaction among amoeba-associated bacteria."
    Schmitz-Esser S., Tischler P., Arnold R., Montanaro J., Wagner M., Rattei T., Horn M.
    J. Bacteriol. 192:1045-1057(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 5a2.

Entry informationi

Entry nameiSYE_AMOA5
AccessioniPrimary (citable) accession number: B3ERQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 22, 2008
Last modified: October 29, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3