Reviewed, UniProtKB/Swiss-Prot B3ERN3 (SYFA_AMOA5)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	Aasi_0475

Organism

Amoebophilus asiaticus (strain 5a2) [Complete proteome] [HAMAP]

Taxonomic identifier

452471 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Candidatus Amoebophilus

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281		PRO_1000114846
Sites
	Metal binding	252	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3ERN3-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 8AA418032215C83F	FASTA	341	39,181
	10 20 30 40 50 60 MLQQLDLIQH EIEQYHPTTL QELEEFRIKF LSKKGTITQL FTEFGQLSPA DKQALGSKLN 70 80 90 100 110 120 ALKQIAQEKY KIYASQLQST PKQNTDVNED YTLPPPADKL GSRHPISILK DRILEIFEKI 130 140 150 160 170 180 GFSIVEGPEI EDDWHNFGAL NFSPNHPARD MLDTFFISQC PDILLRTHTS SVQIRVAESQ 190 200 210 220 230 240 APPIRAISIG RVYRNETISA RSHCMFHQVD AFYVNKNVSF VELKQVLLYF LRSLFGEDIK 250 260 270 280 290 300 MRIRPSYFPF TEPSVEIDIN CRICNGNGCN ICKHSGWLEI MGAGMIDPNV LKNCHIDPTT 310 320 330 340 YTGYAFGMGL ERIAMLMYQI NDLRLFTEND VRFLKQFKAY A

References

[1]

"Complete sequence of Candidatus Amoebophilus asiaticus 5a2." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Horn M., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001102 Genomic DNA. Translation: ACE05885.1.
RefSeq	YP_001957614.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6377145.
GenomeReviews	Gene locus Aasi_0475 in contig CP001102_GR.
KEGG	aas:Aasi_0475.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FRASYFP.
Family and domain databases
HAMAP	MF_00281. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR002319. Phenylalanyl-tRNA_Synthase_acu. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_AMOA5

Accession

Primary (citable) accession number: B3ERN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)