B3ERG6 (UNG_AMOA5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uracil-DNA glycosylase
Short name=UDG
EC=3.2.2.27

Gene names

Name:	ung
Ordered Locus Names:	Aasi_0400

Organism

Amoebophilus asiaticus (strain 5a2) [Complete proteome] [HAMAP]

Taxonomic identifier

452471 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Bacteroidetes › unclassified Bacteroidetes › Candidatus Amoebophilus › Candidatus Amoebophilus asiaticus

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine By similarity. HAMAP-Rule MF_00148
Catalytic activity	Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil. HAMAP-Rule MF_00148
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the uracil-DNA glycosylase family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	base-excision repair Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	uracil DNA N-glycosylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	Uracil-DNA glycosylase HAMAP-Rule MF_00148		PRO_1000096562
Sites
	Active site	65	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3ERG6 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 614A3385FDDB1C8C	FASTA	220	24,992
	10 20 30 40 50 60 MQVNIAPSWK PYLEEEFNKP YFEQLAAFVK AEYKSNTIYP PAKQIFNAFE LCSFDNTKVV 70 80 90 100 110 120 ILGQDPYHGF KQANGLSFSV GKEVRMPPSL VNIFKEIESD LGHPVPTNGD LTRWAQQGVL 130 140 150 160 170 180 LLNATLTVRE KQPGSHQNKG WETFTDTIIS ILSQHKQHLV FMLWGNYAQK KEVLIDTSKH 190 200 210 220 LVLKSAHPSP YAADRGFFGN KHFSKANAYL VEHGLTPINW

References

[1]

"Complete sequence of Candidatus Amoebophilus asiaticus 5a2." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Horn M., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 5a2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001102 Genomic DNA. Translation: ACE05818.1.
RefSeq	YP_001957547.1. NC_010830.1.
3D structure databases
ProteinModelPortal	B3ERG6.
SMR	B3ERG6. Positions 4-220.
ModBase	Search...
Protein-protein interaction databases
STRING	452471.Aasi_0400.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACE05818; ACE05818; Aasi_0400.
GeneID	6377331.
KEGG	aas:Aasi_0400.
PATRIC	21266219. VBICanAmo76781_0464.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0692.
HOGENOM	HOG000229528.
KO	K03648.
OMA	KEPINWK.
Enzyme and pathway databases
BioCyc	AASI452471:GHZ2-439-MONOMER.
Family and domain databases
Gene3D	3.40.470.10. 1 hit.
HAMAP	MF_00148. UDG.
InterPro	IPR018085. Ura-DNA_Glyclase_AS. IPR002043. Ura_DNA_glycsylse. IPR005122. Uracil-DNA_glycosylase-like. [Graphical view]
PANTHER	PTHR11264. PTHR11264. 1 hit.
Pfam	PF03167. UDG. 1 hit. [Graphical view]
SMART	SM00986. UDG. 1 hit. [Graphical view]
SUPFAM	SSF52141. UDNA_glycsylseSF. 1 hit.
TIGRFAMs	TIGR00628. ung. 1 hit.
PROSITE	PS00130. U_DNA_GLYCOSYLASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UNG_AMOA5

Accession

Primary (citable) accession number: B3ERG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	May 1, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program