Reviewed, UniProtKB/Swiss-Prot B3ER47 (MRAY_AMOA5)

Names and origin

Protein names

Recommended name:
    Phospho-N-acetylmuramoyl-pentapeptide-transferase
    EC=2.7.8.13
Alternative name(s):
    UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	Aasi_0256

Organism

Amoebophilus asiaticus (strain 5a2) [Complete proteome] [HAMAP]

Taxonomic identifier

452471 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Candidatus Amoebophilus

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity.
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 404	404	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_1000090588
Regions
	Transmembrane	30 – 50	21	Potential
	Transmembrane	73 – 93	21	Potential
	Transmembrane	100 – 120	21	Potential
	Transmembrane	132 – 152	21	Potential
	Transmembrane	209 – 229	21	Potential
	Transmembrane	242 – 262	21	Potential
	Transmembrane	274 – 294	21	Potential
	Transmembrane	301 – 321	21	Potential
	Transmembrane	326 – 346	21	Potential
	Transmembrane	381 – 401	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	B3ER47-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: EDE0B20118F0E825	FASTA	404	45,209
	10 20 30 40 50 60 MLYYFFKYID RFSHIPGIGV FKYISFRAAS AAILSLCISI FLGKRLIIFF KTAQIKEGIR 70 80 90 100 110 120 ELDLAGQSEK AHIPTMGGII IIAATVVPTL LFAKLKNVYI MLLLISIIWM GLIGFIDDYI 130 140 150 160 170 180 KVFKRDKKGL AGKFKIVGQV ALGVIVGITL IFRDDVVIRE FGSNITVIEN GQVTINDYKD 190 200 210 220 230 240 VKTVKTTIPF LKNNELDYSK LIPWLDSKYM WIVYVLFMIF IIAAVSNGAN LTDGLDGLTA 250 260 270 280 290 300 GTSAIIGTTL AILAYVSGNV IFSRYLNIMY IPNLAELAIF CTAFVGACVG FLWYNTYPAQ 310 320 330 340 350 360 IFMGDTGSLA IGSVIAVLAI VIRKELMIPL LCGIFFIETL SVIIQVGYFK YTKYRYGIGK 370 380 390 400 RMFKMAPLHH HFQKLGFHES KIVTRFWIVG IVLAILSLVT LKLR

References

[1]

"Complete sequence of Candidatus Amoebophilus asiaticus 5a2." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Horn M., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001102 Genomic DNA. Translation: ACE05699.1.
RefSeq	YP_001957428.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6377725.
GenomeReviews	Gene locus Aasi_0256 in contig CP001102_GR.
KEGG	aas:Aasi_0256.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HHHFEQK.
Family and domain databases
HAMAP	MF_00038. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR018481. Glycosyl_Trfase_4_cons-reg. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. PF10555. MraY_sig1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. mraY. 1 hit.
PROSITE	PS01347. MRAY_1. 1 hit. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_AMOA5

Accession

Primary (citable) accession number: B3ER47

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)