B3ER35 (TPIS_AMOA5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Triosephosphate isomerase
Short name=TIM
EC=5.3.1.1

Alternative name(s):
Triose-phosphate isomerase

Gene names

Name:	tpiA
Ordered Locus Names:	Aasi_0244
ORF Names:	Aasi_1507

Organism

Amoebophilus asiaticus (strain 5a2) [Complete proteome] [HAMAP]

Taxonomic identifier

452471 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Candidatus Amoebophilus

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147_B
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147_B Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147_B
Subunit structure	Homodimer By similarity. HAMAP MF_00147_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00147_B.
Sequence similarities	Belongs to the triosephosphate isomerase family.
Sequence caution	The sequence ACP20869.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glycolysis Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Triosephosphate isomerase HAMAP MF_00147_B		PRO_1000096474
Sites
	Active site	98	1	Electrophile By similarity
	Active site	170	1	Proton acceptor By similarity
	Binding site	9	1	Substrate By similarity
	Binding site	11	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3ER35 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: C61ECBA48B6F5229	FASTA	253	28,021
	10 20 30 40 50 60 MQRTIIAANW KMNKNFQEGL QLAKEITQFI QAEPLAGAQI ILFPSFIHLE GISKLLTPEV 70 80 90 100 110 120 KLHLGAQNCH DQIAGAFTGE VSAAMLASID VRYVLVGHSE RRQNFAEDND LIAKKIDAIL 130 140 150 160 170 180 SCKLQPVFCC GEPLSVRESN QHYAFIEQQI AESLFHLTPD ELQQVIIAYE PIWAIGTGLI 190 200 210 220 230 240 PSLAEIEEMQ QTIRNILKKQ YNTVLADNMA ILYGGSCNAS NITKLISLPG INGVLIGGAS 250 LHFKEFIHIL RSL

References

[1]

"Complete sequence of Candidatus Amoebophilus asiaticus 5a2." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Lang D., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Horn M., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 5a2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001102 Genomic DNA. Translation: ACP20869.1. Different initiation.
RefSeq	YP_003572997.1. NC_010830.1.
3D structure databases
ProteinModelPortal	B3ER35.
ModBase	Search...
Protein-protein interaction databases
STRING	B3ER35.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6376715.
GenomeReviews	Gene locus Aasi_0244 in contig CP001102_GR.
KEGG	aas:Aasi_1507.
PATRIC	21265823. VBICanAmo76781_0270.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG708281.
OMA	ANTVIAY.
Family and domain databases
HAMAP	MF_00147_B. TIM_B. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR022896. TrioseP_Isoase_bac/euk. IPR000652. Triosephosphate_isomerase. IPR020861. Triosephosphate_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01803.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
SUPFAM	SSF51351. Triophos_ismrse. 1 hit.
TIGRFAMs	TIGR00419. Tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS_AMOA5

Accession

Primary (citable) accession number: B3ER35
Secondary accession number(s): C3L4F1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program