B3EQC0 (MURD_CHLPB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	Cphamn1_2524

Organism

Chlorobium phaeobacteroides (strain BS1) [Complete proteome] [HAMAP]

Taxonomic identifier

331678 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_1000130839
Regions
	Nucleotide binding	115 – 121	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B3EQC0 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 4029A6233ECF1F8B	FASTA	466	51,157
	10 20 30 40 50 60 MNAETTDGKR VSIIGAKRSG IAAALLLQQR GATVYVSDRE PVGDAERAFL EHHGIAFEEE 70 80 90 100 110 120 GHTDRILEAD FSVVSPGIPP HASVIRRLEH EAIPLFSEIE AASWFCPAFI IGITGTDGKT 130 140 150 160 170 180 TTSSMVEAIC LSGANSREQR VFSAGNIGVP FSSLVGEMRK GDIAVVELSS YQLERCRSFR 190 200 210 220 230 240 PDVAVITNIM PDHLDRYGGS MKRYAEAKYR IYAQQRKQDT LVYNADDETL RAHFEGGGAF 250 260 270 280 290 300 VPQLVPFSLD RKRASLGGAC YAAIEGDWLI TVVNGREEKV ITGSGLFKRS FRGRHNLENA 310 320 330 340 350 360 LAAVAATRAA GVDVFPIREA LRRFAGVEHR QEYVRTMHGV DWINDSKATN LNALRQALDA 370 380 390 400 410 420 TPGKLVLIAG GRDKGDDLSD LEERVRQKVS VLVVFGESKA KFTEAFSSVV RVVPALSLEE 430 440 450 460 AVEQAQRYAS TGETVLFSPG CSSFDMFESF EERGMRFKKH VEGMQS

References

[1]

"Complete sequence of Chlorobium phaeobacteroides BS1." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Li T., Liu Z., Zhao F. , Overmann J., Bryant D.A., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001101 Genomic DNA. Translation: ACE05418.1.
RefSeq	YP_001960899.1. NC_010831.1.
3D structure databases
ProteinModelPortal	B3EQC0.
ModBase	Search...
Protein-protein interaction databases
STRING	B3EQC0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6376222.
GenomeReviews	Gene locus Cphamn1_2524 in contig CP001101_GR.
KEGG	cpb:Cphamn1_2524.
PATRIC	21387527. VBIChlPha121022_2743.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG750024.
OMA	ACASWDM.
ProtClustDB	CLSK2339454.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_CHLPB

Accession

Primary (citable) accession number: B3EQC0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program