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B3EQ14 (DDL_CHLPB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Cphamn1_1004
OrganismChlorobium phaeobacteroides (strain BS1) [Complete proteome] [HAMAP]
Taxonomic identifier331678 [NCBI]
Taxonomic lineageBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 360360D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000091171

Regions

Domain146 – 352207ATP-grasp
Nucleotide binding179 – 23456ATP By similarity

Sites

Metal binding3051Magnesium or manganese 1 By similarity
Metal binding3191Magnesium or manganese 1 By similarity
Metal binding3191Magnesium or manganese 2 By similarity
Metal binding3211Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B3EQ14 [UniParc].

Last modified July 22, 2008. Version 1.
Checksum: F5C669609B815CF1

FASTA36039,787
        10         20         30         40         50         60 
MPNVTVALLF GGKSSEHEIS IISAKAVSAH IDRTTYTLFP LYISRDGRWF KGRTARKVLD 

        70         80         90        100        110        120 
LDITGLLKSR TIETTNRQLL AMTENRDEDL FDFNFQKEGI EVAFPVLHGA YGEDGKIQGL 

       130        140        150        160        170        180 
LEVFAIPYTG CNVQSSSMTM DKEITKLCAV QAGIHVADYM TVLRPDYLNN RSAIAETIKK 

       190        200        210        220        230        240 
RFAPPFFVKP ANLGSSVGIA KIHSFDELEN ALDEACRLDV KILVEKAIEG REVEVAVLGN 

       250        260        270        280        290        300 
EHPIASVPGE IEPGGDFYDF TDKYIDGSAR LHIPARVDAD TSARLQEEGI KAFRALGCSG 

       310        320        330        340        350        360 
MSRIDFFVEK GSGRIILNEI NSIPGFTSIS MYPMLMEHAG IGFTELIDRL VRFALEKTPA 

« Hide

References

[1]"Complete sequence of Chlorobium phaeobacteroides BS1."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Li T., Liu Z., Zhao F. expand/collapse author list , Overmann J., Bryant D.A., Richardson P.
Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BS1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001101 Genomic DNA. Translation: ACE03946.1.
RefSeqYP_001959427.1. NC_010831.1.

3D structure databases

ProteinModelPortalB3EQ14.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING331678.Cphamn1_1004.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACE03946; ACE03946; Cphamn1_1004.
GeneID6374673.
KEGGcpb:Cphamn1_1004.
PATRIC21384170. VBIChlPha121022_1091.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMARSAYGIY.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycCPHA331678:GHME-1031-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_CHLPB
AccessionPrimary (citable) accession number: B3EQ14
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 22, 2008
Last modified: May 14, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways