Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Biotin synthase

Gene

bioB

Organism
Chlorobium phaeobacteroides (strain BS1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi73Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi77Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi80Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi152Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi213Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi283Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCPHA331678:G1GCA-2481-MONOMER
UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:Cphamn1_2458
OrganismiChlorobium phaeobacteroides (strain BS1)
Taxonomic identifieri331678 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium
Proteomesi
  • UP000001228 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003813011 – 339Biotin synthaseAdd BLAST339

Proteomic databases

PRIDEiB3EPW2

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi331678.Cphamn1_2458

Structurei

3D structure databases

ProteinModelPortaliB3EPW2
SMRiB3EPW2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107SFF Bacteria
COG0502 LUCA
HOGENOMiHOG000239958
KOiK01012
OMAiADRFCMG
OrthoDBiPOG091H01DF

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

B3EPW2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVEMNKSIV KAYSVLETGE PLPFDVAVEL AHLAGEDVPD LLSLANKVKN
60 70 80 90 100
RYATLSEGAL HSCSIINAKS GVCAENCRFC AQSLHNSADV EQYSLLDAEA
110 120 130 140 150
IVRSAGDVYD EGIRHFGVVT SGYGYRKVNP EFLKIVAIID QLRQEYPDLN
160 170 180 190 200
VCASLGILGE EPVRMLAEHN VRHYNINIQV APDRYSDLIA DSHPLKDRID
210 220 230 240 250
TIRLLRKYGI PVCCGGILGV GETMRERLDF IYALQELDIA VIPLNVLVPI
260 270 280 290 300
EGTPLQGAAT PALTDIVKTF ALCRLVHPGK IIKFAAGRET VMNDFQGLLM
310 320 330
LAGANGFLTG GYLTTRGRKI GDDREFMRQL AGFAEVSCS
Length:339
Mass (Da):37,070
Last modified:July 22, 2008 - v1
Checksum:iA14E5DA1E9D44D3B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001101 Genomic DNA Translation: ACE05352.1
RefSeqiWP_012475808.1, NC_010831.1

Genome annotation databases

EnsemblBacteriaiACE05352; ACE05352; Cphamn1_2458
KEGGicpb:Cphamn1_2458

Similar proteinsi

Entry informationi

Entry nameiBIOB_CHLPB
AccessioniPrimary (citable) accession number: B3EPW2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 22, 2008
Last modified: May 23, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health