B3EPT5 (PYRE_CHLPB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	Cphamn1_2430

Organism

Chlorobium phaeobacteroides (strain BS1) [Complete proteome] [HAMAP]

Taxonomic identifier

331678 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_1000138772
Regions
	Region	114 – 122	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	118	1	Orotate By similarity
	Binding site	146	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3EPT5 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: D3826980757B49FA	FASTA	193	20,546
	10 20 30 40 50 60 MSTPAIIDIF RSSGALLEGH FKLTSGLHSN TYFQCAKVLQ HPEYLTEICR NIASAFSDVT 70 80 90 100 110 120 IDMVISPAVG GIVVGTETGR QLGVKTIFSE RKNGEMVLRR GFTLSPEEKV LIVEDVITTG 130 140 150 160 170 180 GSVAEVIDIV TKAGAIVAGV GSVVDRSNGK VTLAEKQFSL LCLEVKNYDP DTCPLCAENL 190 PLDAPGSRSL TTS

References

[1]

"Complete sequence of Chlorobium phaeobacteroides BS1." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Li T., Liu Z., Zhao F. , Overmann J., Bryant D.A., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001101 Genomic DNA. Translation: ACE05325.1.
RefSeq	YP_001960806.1. NC_010831.1.
3D structure databases
ProteinModelPortal	B3EPT5.
ModBase	Search...
Protein-protein interaction databases
STRING	B3EPT5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6376125.
GenomeReviews	Gene locus Cphamn1_2430 in contig CP001101_GR.
KEGG	cpb:Cphamn1_2430.
PATRIC	21387323. VBIChlPha121022_2642.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG404341.
OMA	FYLQSAK.
ProtClustDB	PRK00455.
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR023031. Orotate_PribosylTferase. IPR006273. Orotate_PRibTrfase_thermus-typ. IPR000836. PRibTrfase. [Graphical view]
KO	K00762.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01367. PyrE_Therm. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_CHLPB

Accession

Primary (citable) accession number: B3EPT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program