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B3EMN6

- PNP_CHLPB

UniProt

B3EMN6 - PNP_CHLPB

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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Chlorobium phaeobacteroides (strain BS1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi503 – 5031MagnesiumUniRule annotation
Metal bindingi509 – 5091MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciCPHA331678:GHME-574-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Cphamn1_0552
OrganismiChlorobium phaeobacteroides (strain BS1)
Taxonomic identifieri331678 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium
ProteomesiUP000001228: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 734734Polyribonucleotide nucleotidyltransferasePRO_1000147901Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi331678.Cphamn1_0552.

Structurei

3D structure databases

ProteinModelPortaliB3EMN6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini570 – 62960KHUniRule annotationAdd
BLAST
Domaini639 – 71375S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiGTENTIC.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B3EMN6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFIRKEIDLG DGKVITIETG KMAKQADGAT IVRMGDTMVI ATVVSSRTPP
60 70 80 90 100
SPNQDFFPMQ VEYREKYYAA GKFPGGFIKR ESRPSEKEIL SARLIDRALR
110 120 130 140 150
PLFPNGYYQD TQIIISVISS DQLNDADVLG GIAASAAIMV SDIPFANSMS
160 170 180 190 200
EVRVGRINGE FVINPTINEL AQSDIDISIG GTNDTICMLE GEMNEISEAE
210 220 230 240 250
MLEAIRFGHE AIKKICSLQD DIAAEVNKPK RSFLAIDAPD ELKESIKEAC
260 270 280 290 300
EAPLKELAYM PLAKEERAEK TTAVYKSITE QILGRYKNEI TAEDIAADPS
310 320 330 340 350
KALYLNEQII SGHIHAIEKQ VMRHMILDDA KRLDGRKLEE VRPISIELGL
360 370 380 390 400
IPRAHGSALF TRGETQALVV LTLGTKKDAQ MIDTLLDDTE KRFMLHYNFP
410 420 430 440 450
PFSVGETGRV GGVGRREIGH GNLAERAVKK VVPSESEFPY TIRLVSEILE
460 470 480 490 500
SNGSSSMASV CGATLAAMDG GVPLTKPVSG IAMGLIKEDD NYAVLSDILG
510 520 530 540 550
NEDHLGDMDF KVAGTRDGIT ACQMDIKIDG LDYHILEKAL EQSKNGRLHI
560 570 580 590 600
LDKMTESIAD PRGEIGQYAP KLSTIQVPVD AIGMIIGKGG ETIRSITEET
610 620 630 640 650
GAQINVDDDG TVTISSPNGE SAAAAIETIK TLISKPEVGT IYMGKVKDIR
660 670 680 690 700
EDLGAFVEIL PRTDGLVHIS EIARERVNKV SDHLKQGDRV KVKLIDIRKD
710 720 730
QRSGKIRYAL SIKALLDMPA ESANGPSAQE EQNQ
Length:734
Mass (Da):80,167
Last modified:July 22, 2008 - v1
Checksum:i637E7CC1A3BD92ED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001101 Genomic DNA. Translation: ACE03514.1.
RefSeqiYP_001958995.1. NC_010831.1.

Genome annotation databases

EnsemblBacteriaiACE03514; ACE03514; Cphamn1_0552.
GeneIDi6374216.
KEGGicpb:Cphamn1_0552.
PATRICi21383172. VBIChlPha121022_0596.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001101 Genomic DNA. Translation: ACE03514.1 .
RefSeqi YP_001958995.1. NC_010831.1.

3D structure databases

ProteinModelPortali B3EMN6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 331678.Cphamn1_0552.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACE03514 ; ACE03514 ; Cphamn1_0552 .
GeneIDi 6374216.
KEGGi cpb:Cphamn1_0552.
PATRICi 21383172. VBIChlPha121022_0596.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi GTENTIC.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci CPHA331678:GHME-574-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BS1.

Entry informationi

Entry nameiPNP_CHLPB
AccessioniPrimary (citable) accession number: B3EMN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: July 22, 2008
Last modified: November 26, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3