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B3EMN5 (SYL_CHLPB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Cphamn1_0551
OrganismChlorobium phaeobacteroides (strain BS1) [Complete proteome] [HAMAP]
Taxonomic identifier331678 [NCBI]
Taxonomic lineageBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium

Protein attributes

Sequence length806 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 806806Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091304

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif576 – 5805"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5791ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B3EMN5 [UniParc].

Last modified July 22, 2008. Version 1.
Checksum: 10EA7B28DBCFAFC7

FASTA80691,859
        10         20         30         40         50         60 
MRYDFQSIEK KWQAYWNEHQ TFLTKETVDK PKYYVLDMFP YPSGSGLHVG HLEGYTATDI 

        70         80         90        100        110        120 
ISRYKRSRGH NVLHPMGWDA FGLPAEQFAI KTGTHPKVIT EKNVSSFKDT LMRMGFSYDW 

       130        140        150        160        170        180 
NREINTTDPG YYAWTQWIFL QLLDRGLAYT SEIDVNWCEE LKTVLANEEV AEKVADGHAV 

       190        200        210        220        230        240 
VRKPLRQWVL KITAYAERLL SDLDLVDWPE SVKQMQRNWI GRSEGVEIDF QLPCHRTSLK 

       250        260        270        280        290        300 
VYTTRPDTLF GATYLVISPE HPMAEKLATA EHLIEAKNYI EEAKRKTELE RTGLQKEKTG 

       310        320        330        340        350        360 
VFTGSFAVNP ANGQALPVWI SDFVLTSYGT GAIMSVPAHD GRDWEFAKKF DLPIVEVIKS 

       370        380        390        400        410        420 
PHDVEEAVFE EKGSVCINSS NDDISLDGLP FETSFGVMAD WLEKKGTGKR TVKYKLRDWI 

       430        440        450        460        470        480 
FSRQRYWGEP IPVKYYEDGT LRPESDLPLT LPDIEAYEPT TTGESPLANI SEWLYGTDEN 

       490        500        510        520        530        540 
GSFRRETNTM PQWAGSCWYY LRFIDPGNTE KPVDPKKEQY WMNVDLYVGG AEHAVLHLLY 

       550        560        570        580        590        600 
ARFWHKVLFD LGVVSTKEPF QKLFNQGMIL GEDNEKMSKS RGNVIPADQV LGSYGADAVR 

       610        620        630        640        650        660 
LYEMFLGPLE QVKPWNTNGI EGVSRFLSRV WRLIYPEPGS MAELNEAPLT EDLTRTMHKA 

       670        680        690        700        710        720 
VKKITDHTEQ LKFNTAISEM MVFVNELHKS GSRNRTAVET LLLLLSPYAP HICEELWEAI 

       730        740        750        760        770        780 
GHDTSITEEN WPLFDPALAA DNEVTIAVQV NGKLRGTVTA PAGSPKNILL DSARKEESVR 

       790        800 
KFLDGMSIIK EVVVPDRLVN FVVKPG 

« Hide

References

[1]"Complete sequence of Chlorobium phaeobacteroides BS1."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Li T., Liu Z., Zhao F. expand/collapse author list , Overmann J., Bryant D.A., Richardson P.
Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BS1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001101 Genomic DNA. Translation: ACE03513.1.
RefSeqYP_001958994.1. NC_010831.1.

3D structure databases

ProteinModelPortalB3EMN5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING331678.Cphamn1_0551.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACE03513; ACE03513; Cphamn1_0551.
GeneID6374215.
KEGGcpb:Cphamn1_0551.
PATRIC21383168. VBIChlPha121022_0594.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycCPHA331678:GHME-573-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL_CHLPB
AccessionPrimary (citable) accession number: B3EMN5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 22, 2008
Last modified: April 16, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries