B3ELE3 (NADE_CHLPB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	Cphamn1_1813

Organism

Chlorobium phaeobacteroides (strain BS1) [Complete proteome] [HAMAP]

Taxonomic identifier

331678 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000099016
Regions
	Nucleotide binding	36 – 43	8	ATP By similarity
Sites
	Active site	38	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3ELE3 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 1B749BBD2DA3EA3C	FASTA	277	30,969
	10 20 30 40 50 60 MNTQCLHLNY ALVEDILLAF IRNEIRKFGF RSAVFGLSGG IDSAVVCELA KRALGPENVL 70 80 90 100 110 120 AVLMPYRTSS PESVNDARLM VEKTGVRSEE VEITGVVDAF FDGVSGAGNL RMGNVMARAR 130 140 150 160 170 180 MLYLYDISAR DGRLVIGTSN KTELLLGYGT LFGDMASAVN PIGDLYKTQL WGLARHLQIP 190 200 210 220 230 240 EELVSKIPSA DLWEGQSDEA DLGFGYGEVD LLLYMMLEKR MSRSDIIEAG IEESFYDKVR 250 260 270 KMVVRNQYKR MMPVIAKISA RTPGIDFRYA RDWQEVK

References

[1]

"Complete sequence of Chlorobium phaeobacteroides BS1." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Li T., Liu Z., Zhao F. , Overmann J., Bryant D.A., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001101 Genomic DNA. Translation: ACE04731.1.
RefSeq	YP_001960212.1. NC_010831.1.
3D structure databases
ProteinModelPortal	B3ELE3.
ModBase	Search...
Protein-protein interaction databases
STRING	B3ELE3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6375500.
GenomeReviews	Gene locus Cphamn1_1813 in contig CP001101_GR.
KEGG	cpb:Cphamn1_1813.
PATRIC	21385982. VBIChlPha121022_1980.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
OMA	YDISARD.
ProtClustDB	PRK13980.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_CHLPB

Accession

Primary (citable) accession number: B3ELE3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program