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Protein

Nitrogenase iron protein

Gene

nifH

Organism
Chlorobium phaeobacteroides (strain BS1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.UniRule annotation

Catalytic activityi

8 reduced ferredoxin + 8 H+ + N2 + 16 ATP + 16 H2O = 8 oxidized ferredoxin + H2 + 2 NH3 + 16 ADP + 16 phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per dimer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi94Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1
Metal bindingi131Iron-sulfur (4Fe-4S); shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 15ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processNitrogen fixation
Ligand4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCPHA331678:G1GCA-1774-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrogenase iron proteinUniRule annotation (EC:1.18.6.1UniRule annotation)
Alternative name(s):
Nitrogenase Fe proteinUniRule annotation
Nitrogenase component IIUniRule annotation
Nitrogenase reductaseUniRule annotation
Gene namesi
Name:nifHUniRule annotation
Ordered Locus Names:Cphamn1_1751
OrganismiChlorobium phaeobacteroides (strain BS1)
Taxonomic identifieri331678 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium
Proteomesi
  • UP000001228 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002118571 – 274Nitrogenase iron proteinAdd BLAST274

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei97ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferaseUniRule annotation1

Post-translational modificationi

The reversible ADP-ribosylation of Arg-97 inactivates the nitrogenase reductase and regulates nitrogenase activity.UniRule annotation

Keywords - PTMi

ADP-ribosylation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi331678.Cphamn1_1751

Structurei

3D structure databases

ProteinModelPortaliB3EL81
SMRiB3EL81
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NifH/BchL/ChlL family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DSM Bacteria
COG1348 LUCA
HOGENOMiHOG000228826
KOiK02588
OMAiPGDIVCG
OrthoDBiPOG091H0230

Family and domain databases

CDDicd02040 NifH, 1 hit
HAMAPiMF_00533 NifH, 1 hit
InterProiView protein in InterPro
IPR030655 NifH/chlL_CS
IPR000392 NifH/frxC
IPR005977 Nitrogenase_Fe_NifH
IPR027417 P-loop_NTPase
PANTHERiPTHR42864 PTHR42864, 1 hit
PfamiView protein in Pfam
PF00142 Fer4_NifH, 1 hit
PIRSFiPIRSF000363 Nitrogenase_iron, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01287 nifH, 1 hit
PROSITEiView protein in PROSITE
PS00746 NIFH_FRXC_1, 1 hit
PS00692 NIFH_FRXC_2, 1 hit
PS51026 NIFH_FRXC_3, 1 hit

Sequencei

Sequence statusi: Complete.

B3EL81-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKIAIYGKG GIGKSTTTQN TVAGLAEMGK KVMVVGCDPK ADSTRLLLGG
60 70 80 90 100
LTQKTVLDTL REEGEDVELE DIIKEGYGAS RCTESGGPEP GVGCAGRGII
110 120 130 140 150
TSVNMLEQLG AYDDEWGLDY VFYDVLGDVV CGGFAMPIRD GKAEEIYIVV
160 170 180 190 200
SGEMMAMYAA NNICKGILKY ADAGGVRLGG LICNSRKVDN EMEMIQELAR
210 220 230 240 250
QLGTQMIHFV PRDNMVQRAE INRKTVIDFD PAHQQADEYR ALAKKIDDNE
260 270
MFVIPKPLEI EELEKLLIDF GIAN
Length:274
Mass (Da):29,938
Last modified:July 22, 2008 - v1
Checksum:i2EC5324BE528F2D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001101 Genomic DNA Translation: ACE04669.1
RefSeqiWP_012475146.1, NC_010831.1

Genome annotation databases

EnsemblBacteriaiACE04669; ACE04669; Cphamn1_1751
KEGGicpb:Cphamn1_1751

Similar proteinsi

Entry informationi

Entry nameiNIFH_CHLPB
AccessioniPrimary (citable) accession number: B3EL81
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 22, 2008
Last modified: March 28, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health