B3EKU8 (B3EKU8_CHLPB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase HAMAP MF_00012
Short name=DAD HAMAP MF_00012
EC=4.2.1.9 HAMAP MF_00012

Gene names

Name:	ilvD HAMAP MF_00012
Ordered Locus Names:	Cphamn1_1716

Organism

Chlorobium phaeobacteroides (strain BS1) [Complete proteome] [HAMAP]

Taxonomic identifier

331678 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	579 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family. HAMAP MF_00012

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis HAMAP MF_00012
Ligand	4Fe-4S HAMAP MF_00012 Iron Iron-sulfur Metal-binding
Molecular function	Lyase HAMAP MF_00012 EMBL ACE04634.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	133	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012
	Metal binding	206	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012

Sequences

	Sequence		Length	Mass (Da)
	B3EKU8 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 3C095BEF2D20FEB6	FASTA	579	61,476
	10 20 30 40 50 60 MHCSFNHVKK QLMRSDTIKK GFEKAPHRSL LKATGALSSN EDFNKPFIGI CNSFIELIPG 70 80 90 100 110 120 HAHLQKLGQI AKDEIRKAGG VPFEFNTIGV CDGIAMGHLG MRYSLASREL IADSVETVAQ 130 140 150 160 170 180 AHRLDGLVCI PNCDKITPGM MMAAVRMNIP VIFVSGGPMK KGRTPSGETV DLISVFEAVG 190 200 210 220 230 240 RHSTGAISEE ELGVIEDHAC PTCGSCSGMF TANSMNCLCE ALGFALPGNG TIPAVDPRRL 250 260 270 280 290 300 ELVREASRRI IDLVEKDTKP RDILSRESLL NAFALDFAMG GSTNTILHTL AVANEGEVVF 310 320 330 340 350 360 DFSELNDLSA KTPYICKVSP ATQNVHIEDV DRAGGVSAIL KELSRIDGLL DLTRPTVTGK 370 380 390 400 410 420 TLGENIEQAI ITDSSVIRTV ASPYATTGGL AVLYGNLAPE GAVVKTGAVS ESMMNHTGPA 430 440 450 460 470 480 KVYECQDDAL TGIMEGDVVA GDVVVIRYEG PKGGPGMPEM LSPTSAIMGR GLGESVALLT 490 500 510 520 530 540 DGRFSGGSRG ACVGHVSPEA AERGPIAAVR NGDMITIDIP NRTMSVDLPD AAIKERLAQV 550 560 570 PQFVPKITTG YLSRYAQMVT SANTGAILQN PVCKNNKQR

References

[1]

"Complete sequence of Chlorobium phaeobacteroides BS1." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Li T., Liu Z., Zhao F. , Overmann J., Bryant D.A., Richardson P. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001101 Genomic DNA. Translation: ACE04634.1.
RefSeq	YP_001960115.1. NC_010831.1.
3D structure databases
ProteinModelPortal	B3EKU8.
ModBase	Search...
Protein-protein interaction databases
STRING	B3EKU8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6375403.
GenomeReviews	Gene locus Cphamn1_1716 in contig CP001101_GR.
KEGG	cpb:Cphamn1_1716.
PATRIC	21385770. VBIChlPha121022_1874.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK00911.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3EKU8_CHLPB

Accession

Primary (citable) accession number: B3EKU8

Entry history

Integrated into UniProtKB/TrEMBL:	July 22, 2008
Last sequence update:	July 22, 2008
Last modified:	December 14, 2011
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)