B3EIL0 (MURD_CHLL2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	Clim_2504

Organism

Chlorobium limicola (strain DSM 245 / NBRC 103803) [Complete proteome] [HAMAP]

Taxonomic identifier

290315 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 465	465	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_1000130837
Regions
	Nucleotide binding	112 – 118	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B3EIL0 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 024990E5E20DFF26	FASTA	465	50,058
	10 20 30 40 50 60 MDVHGKKASI IGAKRSGVAA AELLAKSGAK VFVSELGEPG ASEAERLADC GIPWEQDGHT 70 80 90 100 110 120 DRVCDADFCV VSPGIPRTAG IIRRVLEQGI PLYSEIEAAS WFCKARIAGI TGTDGKTTTS 130 140 150 160 170 180 TLLHRIAEAD GMKNDYRAFS VGNIGVPFSS LVLDMAPADL AVVELSSYQL EGCETFRPDV 190 200 210 220 230 240 SVITNITPDH LDRYNGDMQL YAAAKFRIYA SQRASDTLVY NFDDPLLRAQ FSGNGRGFPF 250 260 270 280 290 300 RIVPFGIGND VPGSGSEEAF FYDEGMIRHL TAKGISEVFS GEDFLKSSFR GRHNIYNALA 310 320 330 340 350 360 ALAAAAALGI EESVARKAIS GFSGVEHRQE FVCSLDGVEW INDSKATNVN ALLQALDAVP 370 380 390 400 410 420 GSIVLIAGGR DKGNDYTVLF DVVRRKVVTV IAIGEAREKI LQVFEGITRT AGADTLDDAV 430 440 450 460 ALARESAEKG QTVLFSPGCA SFDMFENFEE RGRLFKRSIL ALNPC

References

[1]

"Complete sequence of Chlorobium limicola DSM 245." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Zhao F., Li T., Liu Z. , Overmann J., Bryant D.A., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 245 / NBRC 103803.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001097 Genomic DNA. Translation: ACD91522.1.
RefSeq	YP_001944501.1. NC_010803.1.
3D structure databases
ProteinModelPortal	B3EIL0.
ModBase	Search...
Protein-protein interaction databases
STRING	B3EIL0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6355123.
GenomeReviews	Gene locus Clim_2504 in contig CP001097_GR.
KEGG	cli:Clim_2504.
PATRIC	21377355. VBIChlLim118737_2694.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG750024.
OMA	ACASWDM.
ProtClustDB	PRK02472.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_CHLL2

Accession

Primary (citable) accession number: B3EIL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program