B3EIE2 (B3EIE2_CHLL2) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

Clim_2433

Organism

Chlorobium limicola (strain DSM 245 / NBRC 103803) [Complete proteome] [HAMAP]

Taxonomic identifier

290315 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	798 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding RuleBase RU000518 SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B3EIE2 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 6EDE21C60BA549FD	FASTA	798	89,361
	10 20 30 40 50 60 MASKTEALSA RNRTLIVVES PSKAKTINKY LGDRYTVFAS VGHIKDLPKR EIGLDFDHNY 70 80 90 100 110 120 EPRYEVIAGK EKVVRQLKKL AGEADSVLIA TDPDREGEAI AWHISNEIEA ARKPVFRVLF 130 140 150 160 170 180 NEITKNAILA AISEPRQIDY RLVRSQQTRQ GLDKIVGYKI SPFLWNVVLR GLSAGRVQSV 190 200 210 220 230 240 ALRLICEREE EINRFEIQEY WTVSADFATA KGEIFKAKLV KVDGGKPELS SQEQAEAAAS 250 260 270 280 290 300 LVRNRLYAVG DITAKAQQRK APLPFTTSLL QQAASNQLGF GSQKTMRIAQ QLYEGIDLGS 310 320 330 340 350 360 EGATGLITYM RTDSTRIGSE AVAEAHKYIR AVFGSEYTGY GSSAKSPKNA QDAHEAIRPT 370 380 390 400 410 420 SIERKPEAMK PYLSADQYRL YELIWKRFLA AMMAPAKIEQ TKVDVEDHER RIVFRANGSR 430 440 450 460 470 480 VLFPGFMRVY DDQQELEYEA RTSTKEDVEK EQTVKLPDTL AARDSLNLDE IEQKQSFTRP 490 500 510 520 530 540 PARYSEATLV KDLDNYGIGR PSTYASIFST LQDRRYVVLQ KKKIAPTDLG KDVSQILVAN 550 560 570 580 590 600 FPDIFNIRFT AFMEDELDKV AAGDDEYEKV LDSFYRPLET ALSLRKNDPL IPQNMNAETC 610 620 630 640 650 660 DKCGEGRMII KWTASGKFLG CSRYPACKNI KPISSTKAKP KETGIKCPSC SDGQMLLRDG 670 680 690 700 710 720 RLGPFLACSG YPKCNTLLNL SKQRHIEPLK TPPVQTDLPC PKCGAPLYLR SGKRGLWLGC 730 740 750 760 770 780 SKFPKCRGRL AWNSLDPALQ LHWEGVMAEH RKAHPDVVLT MTDGRPVPMT LPVDDIMARA 790 EENGLIAPVT EENEGVTV

References

[1]

"Complete sequence of Chlorobium limicola DSM 245." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Zhao F., Li T., Liu Z. , Overmann J., Bryant D.A., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 245 / NBRC 103803.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001097 Genomic DNA. Translation: ACD91454.1.
RefSeq	YP_001944433.1. NC_010803.1.
3D structure databases
ProteinModelPortal	B3EIE2.
ModBase	Search...
Protein-protein interaction databases
STRING	B3EIE2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6355904.
GenomeReviews	Gene locus Clim_2433 in contig CP001097_GR.
KEGG	cli:Clim_2433.
PATRIC	21377201. VBIChlLim118737_2618.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG512935.
OMA	EEYWSID.
ProtClustDB	PRK06599.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 3 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B3EIE2_CHLL2

Accession

Primary (citable) accession number: B3EIE2

Entry history

Integrated into UniProtKB/TrEMBL:	July 22, 2008
Last sequence update:	July 22, 2008
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)