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B3EI42

- BIOB_CHLL2

UniProt

B3EI42 - BIOB_CHLL2

Protein

Biotin synthase

Gene

bioB

Organism
Chlorobium limicola (strain DSM 245 / NBRC 103803)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (22 Jul 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi77 – 771Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi80 – 801Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi152 – 1521Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi213 – 2131Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi283 – 2831Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciCLIM290315:GHUH-2481-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Clim_2430
    OrganismiChlorobium limicola (strain DSM 245 / NBRC 103803)
    Taxonomic identifieri290315 [NCBI]
    Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium
    ProteomesiUP000008841: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 336336Biotin synthasePRO_0000381299Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi290315.Clim_2430.

    Structurei

    3D structure databases

    ProteinModelPortaliB3EI42.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OMAiRDFHGMA.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B3EI42-1 [UniParc]FASTAAdd to Basket

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    MTSSVIHQAV IDAYRVLDTG EPLDRETALR LAALPGDAVL DLASLAHKVR    50
    LRYAGEAASL HACSIMNAKS GECGENCRFC AQSRHNSASI DVYDLAGEDA 100
    VLRQAEEAVD SGIPHFGIVT SGYGYLKITP EFNRILGMID MLHRELPGLN 150
    VCASLGVLGE ETSAALAAHG IAHYNINIQV APSRYSDLIA DTHTVVERIA 200
    TVRLLRKHRI PVCCGGILGV GESMTERLEM IFALSELDVS VIPLNVLVPI 250
    DGTPLEGHRT ADIADIVKTF AICRLVHPRT IIKFAAGRET VMKDFQGLLM 300
    LSGANGFLTG GYLTTRGREI DADRELGRQL ESFTEG 336
    Length:336
    Mass (Da):36,276
    Last modified:July 22, 2008 - v1
    Checksum:iB47D2F612627DE15
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001097 Genomic DNA. Translation: ACD91451.1.
    RefSeqiWP_012467316.1. NC_010803.1.
    YP_001944430.1. NC_010803.1.

    Genome annotation databases

    EnsemblBacteriaiACD91451; ACD91451; Clim_2430.
    GeneIDi6355901.
    KEGGicli:Clim_2430.
    PATRICi21377195. VBIChlLim118737_2615.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001097 Genomic DNA. Translation: ACD91451.1 .
    RefSeqi WP_012467316.1. NC_010803.1.
    YP_001944430.1. NC_010803.1.

    3D structure databases

    ProteinModelPortali B3EI42.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 290315.Clim_2430.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACD91451 ; ACD91451 ; Clim_2430 .
    GeneIDi 6355901.
    KEGGi cli:Clim_2430.
    PATRICi 21377195. VBIChlLim118737_2615.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OMAi RDFHGMA.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci CLIM290315:GHUH-2481-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 245 / NBRC 103803.

    Entry informationi

    Entry nameiBIOB_CHLL2
    AccessioniPrimary (citable) accession number: B3EI42
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: July 22, 2008
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3