B3EHP3 (ILVD_CHLL2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Clim_0742

Organism

Chlorobium limicola (strain DSM 245 / NBRC 103803) [Complete proteome] [HAMAP]

Taxonomic identifier

290315 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000089373
Sites
	Metal binding	121	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	194	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	B3EHP3 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: CD09B7A4D4C08186	FASTA	565	59,377
	10 20 30 40 50 60 MRSDTVKTGF EKAPHRSLLK ATGAIASNND FRKPFIGICN SFNELIPGHA HLQELGRIAK 70 80 90 100 110 120 EEVRKAGGVP FEFNTIGVCD GIAMGHIGMR YSLASRELIA DSVETVAEAH RLDGLVCIPN 130 140 150 160 170 180 CDKITPGMMM AALRINIPVI FVSGGPMKAG CTPSGKTVDL ISVFEAVGQC STGEITESEL 190 200 210 220 230 240 ETIQDSACPG CGSCSGMFTA NSMNCLSEAL GIALPGNGTI LAIDPRRNEL VREASRKIVD 250 260 270 280 290 300 LVNNDIRPRD IITRKSLLNA FALDFAMGGS TNTILHTLAI ANEAELDFDF SELNALSAKT 310 320 330 340 350 360 PYICKVSPAT MDVHIEDVDR AGGISAILKE LSRVDGLLDL SAPTVTGKTL GENIAGAEVL 370 380 390 400 410 420 DRNVIRSIEN PYSATGGLAV LYGNLAPQGA VIKTGAVSPE MMTHTGPAKV YDSQDEAIKG 430 440 450 460 470 480 IMDGDICAGD VVVIRYEGPK GGPGMPEMLS PTSAIMGRGL GGSVALITDG RFSGGSRGAC 490 500 510 520 530 540 IGHVSPEAAE KGPIAALENG DMITIDIPNR CISVDLPETV IAGRIAALKP FEPKIKKGYL 550 560 ARYAQLVTSA NTGAIMKNPA YCESK

References

[1]

"Complete sequence of Chlorobium limicola DSM 245." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Zhao F., Li T., Liu Z. , Overmann J., Bryant D.A., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 245 / NBRC 103803.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001097 Genomic DNA. Translation: ACD89823.1.
RefSeq	YP_001942802.1. NC_010803.1.
3D structure databases
ProteinModelPortal	B3EHP3.
ModBase	Search...
Protein-protein interaction databases
STRING	B3EHP3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6356023.
GenomeReviews	Gene locus Clim_0742 in contig CP001097_GR.
KEGG	cli:Clim_0742.
PATRIC	21373487. VBIChlLim118737_0786.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK00911.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_CHLL2

Accession

Primary (citable) accession number: B3EHP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program