Reviewed, UniProtKB/Swiss-Prot B3EH29 (DAPA_CHLL2)

Names and origin

Protein names

Recommended name:
    Dihydrodipicolinate synthase
      Short name=DHDPS
    EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	Clim_0622

Organism

Chlorobium limicola (strain DSM 245 / NBRC 103803) [Complete proteome] [HAMAP]

Taxonomic identifier

290315 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_1000124021
Regions
	Region	52 – 53	2	Pyruvate binding By similarity
Sites
	Active site	166	1	Schiff-base intermediate with substrate By similarity
	Binding site	110	1	Pyruvate By similarity
	Site	137	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3EH29-1 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: F105BE73DA3C03D2	FASTA	296	32,025
	10 20 30 40 50 60 MSTRLISGSA VALVTPFRQD CSVDTVALRQ LVRFHIAAGT DIIIPCGTTG ESPTLSMEEQ 70 80 90 100 110 120 SRIIRTVKEE AGEQIMVAAG AGTNATDHAV ELAKNAEKAG ASAILSVAPY YNKPSQEGIY 130 140 150 160 170 180 QHYARIAEAV SVPIIIYNVP GRTGSNVAAS TILRLARDFG NIAAVKEASD NMAQITELLE 190 200 210 220 230 240 ERPENFSVLT GEDMLILPFM AMGGDGVISV AANQVPSAVK RLVEAARTGR LDEARAINTR 250 260 270 280 290 YRRLFRLNFI ESNPVPVKYA LALMGMIEEV YRLPLVPLAD ENKRILKEEL QSLGLV

References

[1]

"Complete sequence of Chlorobium limicola DSM 245." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Zhao F., Li T., Liu Z. , Overmann J., Bryant D.A., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001097 Genomic DNA. Translation: ACD89709.1.
RefSeq	YP_001942688.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6354070.
GenomeReviews	Gene locus Clim_0622 in contig CP001097_GR.
KEGG	cli:Clim_0622.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VAPRLMH.
Family and domain databases
HAMAP	MF_00418. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005263. DapA_synth. IPR002220. DHDPS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. dapA. 1 hit.
PROSITE	PS00665. DHDPS_1. False negative. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_CHLL2

Accession

Primary (citable) accession number: B3EH29

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 22, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)