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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Chlorobium limicola (strain DSM 245 / NBRC 103803)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi506 – 5061MagnesiumUniRule annotation
Metal bindingi512 – 5121MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciCLIM290315:GHUH-627-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Clim_0599
OrganismiChlorobium limicola (strain DSM 245 / NBRC 103803)
Taxonomic identifieri290315 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium
ProteomesiUP000008841: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 736736Polyribonucleotide nucleotidyltransferasePRO_0000381873Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi290315.Clim_0599.

Structurei

3D structure databases

ProteinModelPortaliB3EH06.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini573 – 63260KHUniRule annotationAdd
BLAST
Domaini642 – 71776S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B3EH06-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MISMIINKAI DLGQGKIISI ETGKMAKQAD GSVVVRLGDT MVLATVVSSK
60 70 80 90 100
KTPPPNQDYF PLQVEYREKY SAAGKFPGGF FKRESRPSEK EILSARLIDR
110 120 130 140 150
ALRPLFPDGY LFETQIIVTV ISSDQINDAD VLGGLAASAA IMVSDIPFHN
160 170 180 190 200
AMSEVRVGRI NGKFIINPDV NELVNSDLDI CIGGTTDTIC MLEGEMKEIS
210 220 230 240 250
EAEMLDAIKF GHDAIRRLCA LQNEIAAEVA KPARPFAPAV IPSELTDLVR
260 270 280 290 300
GLCETRLREL AYTPLRKEDR AEQTAAIYRE IIQSTVEHFK AAISPEEIQA
310 320 330 340 350
DPSKALCLNE HIIDEQIHAV EKKVMRHMIL DDAKRLDGRT LEEVRPISIE
360 370 380 390 400
LGIIPRAHGS ALFTRGETQA LVTITLGTKK DAQSVDNLTN NADKKFMLHY
410 420 430 440 450
NFPPFSVGET GRIGSTGRRE IGHGNLAERA IKMVAPPEQE FPYTVRIVSD
460 470 480 490 500
ILESNGSSSM ASVCGGTLAL MDGGVPIRKP VSGIAMGLIK EGSNYAVLSD
510 520 530 540 550
ILGNEDHLGD MDFKVSGTRD GITACQMDIK IDGLDYHILE SALEQARRGR
560 570 580 590 600
LHILDKMEEA IPSARVELAQ FAPRLTTIQV PVDAIGLIIG KGGETIRSIT
610 620 630 640 650
EETGAEINIE DDGTVTIACS SVEGTHAALA TIKTLLAKPE VGTIYLGKVR
660 670 680 690 700
DVRDELGAFV EFLPKTDGLV HISEISATER VAKVSDHLKI GDRIKVKLVD
710 720 730
VRKDPRTGKT RFALSMRAVE TDAANGAPQE NNQENQ
Length:736
Mass (Da):80,188
Last modified:July 22, 2008 - v1
Checksum:iA7748BE947B94D2E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001097 Genomic DNA. Translation: ACD89686.1.
RefSeqiWP_012465567.1. NC_010803.1.
YP_001942665.1. NC_010803.1.

Genome annotation databases

EnsemblBacteriaiACD89686; ACD89686; Clim_0599.
GeneIDi6354047.
KEGGicli:Clim_0599.
PATRICi21373193. VBIChlLim118737_0639.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001097 Genomic DNA. Translation: ACD89686.1.
RefSeqiWP_012465567.1. NC_010803.1.
YP_001942665.1. NC_010803.1.

3D structure databases

ProteinModelPortaliB3EH06.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290315.Clim_0599.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD89686; ACD89686; Clim_0599.
GeneIDi6354047.
KEGGicli:Clim_0599.
PATRICi21373193. VBIChlLim118737_0639.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciCLIM290315:GHUH-627-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 245 / NBRC 103803.

Entry informationi

Entry nameiPNP_CHLL2
AccessioniPrimary (citable) accession number: B3EH06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: July 22, 2008
Last modified: January 7, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.