Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Chlorobium limicola (strain DSM 245 / NBRC 103803)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi7 – 71MagnesiumUniRule annotation
Metal bindingi67 – 671MagnesiumUniRule annotation
Metal bindingi140 – 1401MagnesiumUniRule annotation
Metal bindingi143 – 1431MagnesiumUniRule annotation

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. nucleic acid binding Source: InterPro

GO - Biological processi

  1. DNA recombination Source: UniProtKB-HAMAP
  2. DNA repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciCLIM290315:GHUH-498-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:Clim_0476
OrganismiChlorobium limicola (strain DSM 245 / NBRC 103803)
Taxonomic identifieri290315 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium
ProteomesiUP000008841: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 190190Crossover junction endodeoxyribonuclease RuvCPRO_1000090512Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi290315.Clim_0476.

Structurei

3D structure databases

ProteinModelPortaliB3EG36.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012181.
KOiK01159.
OMAiYTALQMK.
OrthoDBiEOG6RG044.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B3EG36-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVVLGVDPGS LHTGYGVIRY DNAGFHLLGC GVIRLHRRKS YFERIGEIYR
60 70 80 90 100
ELDSVIGGYA PVRLALETVF LNKNVQSALK LGQVRGAVIA LAMNHRLELS
110 120 130 140 150
EYAPREVKAA VTGRGAASKE QVAFMVTRML KITGNPATHD VTDALAIALC
160 170 180 190
DLLKTGMGSA AVPQSVVSSG SAGKKNWAQF VQSNPGMVVR
Length:190
Mass (Da):20,380
Last modified:July 22, 2008 - v1
Checksum:i4C377F244D15A6AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001097 Genomic DNA. Translation: ACD89569.1.
RefSeqiWP_012465450.1. NC_010803.1.
YP_001942548.1. NC_010803.1.

Genome annotation databases

EnsemblBacteriaiACD89569; ACD89569; Clim_0476.
GeneIDi6354471.
KEGGicli:Clim_0476.
PATRICi21372927. VBIChlLim118737_0512.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001097 Genomic DNA. Translation: ACD89569.1.
RefSeqiWP_012465450.1. NC_010803.1.
YP_001942548.1. NC_010803.1.

3D structure databases

ProteinModelPortaliB3EG36.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290315.Clim_0476.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACD89569; ACD89569; Clim_0476.
GeneIDi6354471.
KEGGicli:Clim_0476.
PATRICi21372927. VBIChlLim118737_0512.

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012181.
KOiK01159.
OMAiYTALQMK.
OrthoDBiEOG6RG044.

Enzyme and pathway databases

BioCyciCLIM290315:GHUH-498-MONOMER.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 245 / NBRC 103803.

Entry informationi

Entry nameiRUVC_CHLL2
AccessioniPrimary (citable) accession number: B3EG36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 22, 2008
Last modified: February 4, 2015
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.