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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Chlorobium limicola (strain DSM 245 / NBRC 103803 / 6330)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi7MagnesiumUniRule annotation1
Metal bindingi67MagnesiumUniRule annotation1
Metal bindingi140MagnesiumUniRule annotation1
Metal bindingi143MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Nuclease
Biological processDNA damage, DNA recombination, DNA repair
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciCLIM290315:G1GC7-495-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:Clim_0476
OrganismiChlorobium limicola (strain DSM 245 / NBRC 103803 / 6330)
Taxonomic identifieri290315 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium
Proteomesi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000905121 – 190Crossover junction endodeoxyribonuclease RuvCAdd BLAST190

Proteomic databases

PRIDEiB3EG36

Interactioni

Protein-protein interaction databases

STRINGi290315.Clim_0476

Structurei

3D structure databases

ProteinModelPortaliB3EG36
SMRiB3EG36
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105NHU Bacteria
COG0817 LUCA
HOGENOMiHOG000012181
KOiK01159
OMAiGLTRCGI
OrthoDBiPOG091H0202

Family and domain databases

Gene3Di3.30.420.10, 1 hit
HAMAPiMF_00034 RuvC, 1 hit
InterProiView protein in InterPro
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
IPR020563 X-over_junc_endoDNase_Mg_BS
IPR002176 X-over_junc_endoDNase_RuvC
PfamiView protein in Pfam
PF02075 RuvC, 1 hit
PRINTSiPR00696 RSOLVASERUVC
SUPFAMiSSF53098 SSF53098, 1 hit
TIGRFAMsiTIGR00228 ruvC, 1 hit
PROSITEiView protein in PROSITE
PS01321 RUVC, 1 hit

Sequencei

Sequence statusi: Complete.

B3EG36-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVLGVDPGS LHTGYGVIRY DNAGFHLLGC GVIRLHRRKS YFERIGEIYR
60 70 80 90 100
ELDSVIGGYA PVRLALETVF LNKNVQSALK LGQVRGAVIA LAMNHRLELS
110 120 130 140 150
EYAPREVKAA VTGRGAASKE QVAFMVTRML KITGNPATHD VTDALAIALC
160 170 180 190
DLLKTGMGSA AVPQSVVSSG SAGKKNWAQF VQSNPGMVVR
Length:190
Mass (Da):20,380
Last modified:July 22, 2008 - v1
Checksum:i4C377F244D15A6AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001097 Genomic DNA Translation: ACD89569.1
RefSeqiWP_012465450.1, NC_010803.1

Genome annotation databases

EnsemblBacteriaiACD89569; ACD89569; Clim_0476
KEGGicli:Clim_0476

Entry informationi

Entry nameiRUVC_CHLL2
AccessioniPrimary (citable) accession number: B3EG36
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 22, 2008
Last modified: May 23, 2018
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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