B3EG23 (LPXK_CHLL2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tetraacyldisaccharide 4'-kinase
EC=2.7.1.130

Alternative name(s):
Lipid A 4'-kinase

Gene names

Name:	lpxK
Ordered Locus Names:	Clim_0463

Organism

Chlorobium limicola (strain DSM 245 / NBRC 103803) [Complete proteome] [HAMAP]

Taxonomic identifier

290315 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transfers the gamma-phosphate of ATP to the 4'-position of a tetraacyldisaccharide 1-phosphate intermediate (termed DS-1-P) to form tetraacyldisaccharide 1,4'-bis-phosphate (lipid IVA) By similarity. HAMAP MF_00409
Catalytic activity	ATP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate) = ADP + (2-N,3-O-bis(3-hydroxytetradecanoyl)-4-O-phosphono-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate). HAMAP MF_00409
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 6/6. HAMAP MF_00409
Sequence similarities	Belongs to the LpxK family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tetraacyldisaccharide 4'-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	Tetraacyldisaccharide 4'-kinase HAMAP MF_00409		PRO_1000191527
Regions
	Nucleotide binding	48 – 55	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B3EG23 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 67709B3BAB0EA53F	FASTA	350	38,464
	10 20 30 40 50 60 MSNPLSPLLK PAAALYRTVV RMRNLGFEKK LFKTWKAPLP VVSIGNISAG GTGKTPLADW 70 80 90 100 110 120 IINYCLSVGS EPALLSRGYG RTTKGVQLVS DGQRILLDSR EAGDETSMLA ARNPGIIVVV 130 140 150 160 170 180 AEKRKEGVEF ILKRFGTRMP SLIILDDAFQ HRQIARDLDI VIINAAEPYC NARMLPEGRL 190 200 210 220 230 240 REPLGNIRRA GLIVLNKITD RNAADAIACD LKKTGIPVVL AKTEAGELVP FGEDAGERNM 250 260 270 280 290 300 NGIRAFAFAG IGSPEGFLGS LKEKGIQVEA HRFFRDHEPY SGDKLLPILL EAEKKGLSLV 310 320 330 340 350 TTEKDYFRLL GEHELTATLS VLPCYYLKIS TRFLEGEEIL ASMLNKVIFS

References

[1]

"Complete sequence of Chlorobium limicola DSM 245." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Zhao F., Li T., Liu Z. , Overmann J., Bryant D.A., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 245 / NBRC 103803.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001097 Genomic DNA. Translation: ACD89556.1.
RefSeq	YP_001942535.1. NC_010803.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B3EG23.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6354458.
GenomeReviews	Gene locus Clim_0463 in contig CP001097_GR.
KEGG	cli:Clim_0463.
PATRIC	21372901. VBIChlLim118737_0499.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG663914.
OMA	VILMDDG.
ProtClustDB	CLSK637887.
Family and domain databases
HAMAP	MF_00409. LpxK. [Tree]
InterPro	IPR003758. Tetraacyldisaccharide_4-kinase. [Graphical view]
KO	K00912.
Pfam	PF02606. LpxK. 1 hit. [Graphical view]
TIGRFAMs	TIGR00682. LpxK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXK_CHLL2

Accession

Primary (citable) accession number: B3EG23

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program