B3EEM7 (LSPA_CHLL2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein signal peptidase
EC=3.4.23.36

Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	Clim_1798

Organism

Chlorobium limicola (strain DSM 245 / NBRC 103803) [Complete proteome] [HAMAP]

Taxonomic identifier

290315 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	168 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity. HAMAP MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_00161.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 168	168	Lipoprotein signal peptidase HAMAP MF_00161		PRO_1000097246
Regions
	Transmembrane	57 – 77	21	Helical; Potential
	Transmembrane	85 – 105	21	Helical; Potential
	Transmembrane	130 – 150	21	Helical; Potential
Sites
	Active site	103	1	By similarity
	Active site	138	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3EEM7 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: BCB6C95CDF30D898	FASTA	168	18,826
	10 20 30 40 50 60 MKWFFTFASV VVLLDQFTKK LAVLFLRDRG TVTIIPDWLK LTYAENNGIA FGVEFASQAI 70 80 90 100 110 120 MILLVGSISL MIALYVLKSG NRKTLFLLPF SLIFGGGIGN LIDRLTVGRV IDFIHFDLYQ 130 140 150 160 GTIMGSWVSL WPIFNVADSA ITIGACMLIL LHNRIFPEPD TKAENHVR

References

[1]

"Complete sequence of Chlorobium limicola DSM 245." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Zhao F., Li T., Liu Z. , Overmann J., Bryant D.A., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 245 / NBRC 103803.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001097 Genomic DNA. Translation: ACD90837.1.
RefSeq	YP_001943816.1. NC_010803.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B3EEM7.
Protein family/group databases
MEROPS	A08.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6354627.
GenomeReviews	Gene locus Clim_1798 in contig CP001097_GR.
KEGG	cli:Clim_1798.
PATRIC	21375811. VBIChlLim118737_1928.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG724422.
OMA	VIVVIAR.
ProtClustDB	PRK14787.
Family and domain databases
HAMAP	MF_00161. LspA. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
KO	K03101.
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. LspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_CHLL2

Accession

Primary (citable) accession number: B3EEM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program