B3EEA0 (KATG_CHLL2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Clim_0134

Organism

Chlorobium limicola (strain DSM 245 / NBRC 103803) [Complete proteome] [HAMAP]

Taxonomic identifier

290315 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	732 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 732	732	Catalase-peroxidase HAMAP MF_01961		PRO_0000354758
Sites
	Active site	98	1	Proton acceptor By similarity
	Metal binding	261	1	Iron (heme axial ligand) By similarity
	Site	94	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	97 ↔ 220		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-246) By similarity
	Cross-link	220 ↔ 246		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-97) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3EEA0 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 9E5FCAEE9CD5B544	FASTA	732	81,360
	10 20 30 40 50 60 MSEQSKCPVT GRTAGHPVAG GGMSNRDWWP NQLPLDMLHQ HSSLVNPMGE AFRYKEEFKK 70 80 90 100 110 120 LDLGAVKKDL YALMTDSQEW WPADYGHYGG LFIRMAWHSA GTYRTSDGRG GGGTGNQRFA 130 140 150 160 170 180 PLNSWPDNAN LDKARRLLWP IKQKYGKMLS WADLMILAGN CALESMGFRT FGFGGGRVDI 190 200 210 220 230 240 WEPEEDIYWG KEVEWLGSNR YSGERDLENP LAAVQMGLIY VNPEGPDGNP DPVAAGRDIR 250 260 270 280 290 300 ETFARMAMND EETVALVAGG HTFGKCHGVG DPNLIGPEPE AAPIEEQGLG WKSGYGSGKG 310 320 330 340 350 360 DETMTSGLEG AWTPDPIHWD MGYLGMLFKY EWELTKSPAG AWQWKPKDVA EEDLAPAAHD 370 380 390 400 410 420 PSKRVPTMMT TADLAMRMDP IYGPISRRYY EHPDQFADAF ARAWFKLTHR DMGPKSRYLG 430 440 450 460 470 480 AEVPAEDLIW QDPVPAVDHE LITEGEIAEL KMRILASGLP IPELVSTAWA SASTFRGSDK 490 500 510 520 530 540 RGGANGARIR LAPQKEWEVN QPEQLQRVLH ELEKIRDTFN GEQSGGKRVS LADLIVLGGC 550 560 570 580 590 600 AAVEEAARRA GHDVTVPFAP GRTDASQAET DVESFAALEP LADGFRNYAK RKYSVTPEEM 610 620 630 640 650 660 LIDRSQLLTL TAPEMTVLVG GLRVLGINFR QSPHGVFTRH PETLTNDFFV NLLDMGTEWK 670 680 690 700 710 720 PVSHEHDTFE GRDRKTGEPS WSATRVDLIF GSNARLRAIA EVYGSDDAQE KFVHDFVAAW 730 DKVMNLDRFD LS

References

[1]

"Complete sequence of Chlorobium limicola DSM 245." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Zhao F., Li T., Liu Z. , Overmann J., Bryant D.A., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 245 / NBRC 103803.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001097 Genomic DNA. Translation: ACD89234.1.
RefSeq	YP_001942213.1. NC_010803.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B3EEA0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6356099.
GenomeReviews	Gene locus Clim_0134 in contig CP001097_GR.
KEGG	cli:Clim_0134.
PATRIC	21372175. VBIChlLim118737_0151.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
OMA	WPNALNL.
ProtClustDB	PRK15061.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_CHLL2

Accession

Primary (citable) accession number: B3EEA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program