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B3EE40 (ISPE_CHLL2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Short name=CMK
EC=2.7.1.148
Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase
Gene names
Name:ispE
Ordered Locus Names:Clim_1700
OrganismChlorobium limicola (strain DSM 245 / NBRC 103803) [Complete proteome] [HAMAP]
Taxonomic identifier290315 [NCBI]
Taxonomic lineageBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium

Protein attributes

Sequence length288 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP-Rule MF_00061

Catalytic activity

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP-Rule MF_00061

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP-Rule MF_00061

Sequence similarities

Belongs to the GHMP kinase family. IspE subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2882884-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP-Rule MF_00061
PRO_1000092070

Regions

Nucleotide binding93 – 10311ATP Potential

Sites

Active site111 By similarity
Active site1351 By similarity

Sequences

Sequence LengthMass (Da)Tools
B3EE40 [UniParc].

Last modified July 22, 2008. Version 1.
Checksum: B6C39F72B9529274

FASTA28831,582
        10         20         30         40         50         60 
MLPVSVKSFA KINLGLLITS KRSDGYHTLE TVFAPIDWYD TLEFSPSDTL SMCCTDSALP 

        70         80         90        100        110        120 
VDENNLCMRA AKALQESAGC STGVSITLDK RIPFGAGLGG GSSDAATVLG MLNEFWNVRA 

       130        140        150        160        170        180 
SLADLHILAV RLGADVPYFL EMKGLAFAKG IGDELEDLSL RLPFHIVTVF PEVHISTVWA 

       190        200        210        220        230        240 
YKHFYPRFER TSPDLRPLVT ALCLEGERSC LGAFENDFEP AVFDHYPGVK KVKQDLLDSG 

       250        260        270        280 
SFFASLSGSG SAVFGFFDKR EDADDALMMM REQGFRTSIT APDFSMVR 

« Hide

References

[1]"Complete sequence of Chlorobium limicola DSM 245."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Zhao F., Li T., Liu Z. expand/collapse author list , Overmann J., Bryant D.A., Richardson P.
Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 245 / NBRC 103803.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001097 Genomic DNA. Translation: ACD90742.1.
RefSeqYP_001943721.1. NC_010803.1.

3D structure databases

ProteinModelPortalB3EE40.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290315.Clim_1700.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACD90742; ACD90742; Clim_1700.
GeneID6354007.
KEGGcli:Clim_1700.
PATRIC21375601. VBIChlLim118737_1824.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1947.
HOGENOMHOG000019601.
KOK00919.
OMAFAPINWY.
OrthoDBEOG62VNQ2.

Enzyme and pathway databases

BioCycCLIM290315:GHUH-1745-MONOMER.
UniPathwayUPA00056; UER00094.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPMF_00061. IspE.
InterProIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR004424. IspE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERPTHR20861:SF2. PTHR20861:SF2. 1 hit.
PfamPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFPIRSF010376. IspE. 1 hit.
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsTIGR00154. ispE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameISPE_CHLL2
AccessionPrimary (citable) accession number: B3EE40
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 22, 2008
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways