B3EE40 (ISPE_CHLL2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Clim_1700

Organism

Chlorobium limicola (strain DSM 245 / NBRC 103803) [Complete proteome] [HAMAP]

Taxonomic identifier

290315 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium › Chlorobium limicola

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP-Rule MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP-Rule MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP-Rule MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: UniProtKB-UniPathway terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP-Rule MF_00061		PRO_1000092070
Regions
	Nucleotide binding	93 – 103	11	ATP Potential
Sites
	Active site	11	1	By similarity
	Active site	135	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3EE40 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: B6C39F72B9529274	FASTA	288	31,582
	10 20 30 40 50 60 MLPVSVKSFA KINLGLLITS KRSDGYHTLE TVFAPIDWYD TLEFSPSDTL SMCCTDSALP 70 80 90 100 110 120 VDENNLCMRA AKALQESAGC STGVSITLDK RIPFGAGLGG GSSDAATVLG MLNEFWNVRA 130 140 150 160 170 180 SLADLHILAV RLGADVPYFL EMKGLAFAKG IGDELEDLSL RLPFHIVTVF PEVHISTVWA 190 200 210 220 230 240 YKHFYPRFER TSPDLRPLVT ALCLEGERSC LGAFENDFEP AVFDHYPGVK KVKQDLLDSG 250 260 270 280 SFFASLSGSG SAVFGFFDKR EDADDALMMM REQGFRTSIT APDFSMVR

References

[1]

"Complete sequence of Chlorobium limicola DSM 245." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Zhao F., Li T., Liu Z. , Overmann J., Bryant D.A., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 245 / NBRC 103803.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001097 Genomic DNA. Translation: ACD90742.1.
RefSeq	YP_001943721.1. NC_010803.1.
3D structure databases
ProteinModelPortal	B3EE40.
ModBase	Search...
Protein-protein interaction databases
STRING	290315.Clim_1700.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACD90742; ACD90742; Clim_1700.
GeneID	6354007.
KEGG	cli:Clim_1700.
PATRIC	21375601. VBIChlLim118737_1824.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HOG000019601.
KO	K00919.
OMA	CVYSETA.
ProtClustDB	PRK14616.
Enzyme and pathway databases
BioCyc	CLIM290315:GHUH-1745-MONOMER.
UniPathway	UPA00056; UER00094.
Family and domain databases
Gene3D	3.30.230.10. 1 hit.
HAMAP	MF_00061. IspE.
InterPro	IPR013750. GHMP_kinase_C_dom. IPR006204. GHMP_kinase_N_dom. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
PANTHER	PTHR20861:SF2. PTHR20861:SF2. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. ispE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_CHLL2

Accession

Primary (citable) accession number: B3EE40

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program