B3EDE5 (SYD_CHLL2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	Clim_1519

Organism

Chlorobium limicola (strain DSM 245 / NBRC 103803) [Complete proteome] [HAMAP]

Taxonomic identifier

290315 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	605 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 605	605	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_1000090975

Sequences

	Sequence		Length	Mass (Da)
	B3EDE5 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 9134C88D35928130	FASTA	605	69,203
	10 20 30 40 50 60 MYRAEESMMP LENRFRTDYC GLLGVGSENR SVRLGGWVHR KRDHGGLIFI DLRDHTGVCQ 70 80 90 100 110 120 LVIQPEQQAL FNAVEHLHAE SVICIEGTVV LRSSETVNTR LASGEIEVVV SSVIIESDAQ 130 140 150 160 170 180 PLPFPVADEV PTSEELRLKY RFIDLRREKI HENIIFRSRL TAMVRRYLEE REFIEIQTPI 190 200 210 220 230 240 LTSSSPEGAR DFLVPSRLHP GKFYALPQAP QQFKQLLMVS GFPRYFQIAP CFRDEDARAD 250 260 270 280 290 300 RSPGEFYQID MEMAFIEQND LFEILEGMFK HLTENMSQKR ITQFPFPRIS YREVMNRFGS 310 320 330 340 350 360 DKPDLRIPLE IQDVTSLFVN SSFKVFASNT KEGTCVKALV LKGRGGESRM FYDKAEKRAR 370 380 390 400 410 420 ELGSAGLAYI QFREEGPKGP IVKFLGEAEM EVLKEQLGLE TGDVVFFGAG KWESTCKIMG 430 440 450 460 470 480 GMRIYFADLF DLDKDELSFC WIVDFPMYEY NEEAKKIDFS HNPFSMPQGE MEALETMQPL 490 500 510 520 530 540 DILAYQYDIV CNGIELSSGA IRNHRPDIMY RAFEIAGYTK EEVDSRFGHM IDAFKLGAPP 550 560 570 580 590 600 HGGIAPGLDR MVMILRDEQN IREVIAFPMN QQAQDLMMSA PSEVMAQQLK ELHLKIDLPP VKEAK

References

[1]

"Complete sequence of Chlorobium limicola DSM 245." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Zhao F., Li T., Liu Z. , Overmann J., Bryant D.A., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 245 / NBRC 103803.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001097 Genomic DNA. Translation: ACD90570.1.
RefSeq	YP_001943549.1. NC_010803.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B3EDE5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6355776.
GenomeReviews	Gene locus Clim_1519 in contig CP001097_GR.
KEGG	cli:Clim_1519.
PATRIC	21375175. VBIChlLim118737_1618.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG396032.
OMA	YQLDVEM.
ProtClustDB	PRK00476.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_CHLL2

Accession

Primary (citable) accession number: B3EDE5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program