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B3ED46 (AMPA_CHLL2) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable cytosol aminopeptidase

EC=3.4.11.1
Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase
Gene names
Name:pepA
Ordered Locus Names:Clim_1411
OrganismChlorobium limicola (strain DSM 245 / NBRC 103803) [Complete proteome] [HAMAP]
Taxonomic identifier290315 [NCBI]
Taxonomic lineageBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium

Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181

Catalytic activity

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181

Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Cofactor

Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181

Subcellular location

Cytoplasm By similarity HAMAP MF_00181.

Sequence similarities

Belongs to the peptidase M17 family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandManganese
Metal-binding
   Molecular functionAminopeptidase
Hydrolase
Protease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionaminopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-KW

manganese ion binding

Inferred from electronic annotation. Source: InterPro

metalloexopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 503503Probable cytosol aminopeptidase HAMAP MF_00181
PRO_1000098313

Sites

Active site2841 Potential
Active site3581 Potential
Metal binding2721Manganese 2 By similarity
Metal binding2771Manganese 1 By similarity
Metal binding2771Manganese 2 By similarity
Metal binding2951Manganese 2 By similarity
Metal binding3541Manganese 1 By similarity
Metal binding3561Manganese 1 By similarity
Metal binding3561Manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B3ED46 [UniParc].

Last modified July 22, 2008. Version 1.
Checksum: 3FF1B29A68372BB7

FASTA50352,760
        10         20         30         40         50         60 
MNISVTATPV KKIKTELLVV PFTTGALKKN ADGILQDLGY DAVVLRDFKA DAGELVILYG 

        70         80         90        100        110        120 
AAGKAIAARA ALLGMGEGKK VTDFRKAAAA LALKAMDMKI ESVAVDFSGV KGFASSAKSS 

       130        140        150        160        170        180 
VASICSAFIE GCYTGSYRFD RLKSDKLKKK KDESDKTKEI SELVLRAEPA QLSAVEDGLA 

       190        200        210        220        230        240 
AGIITGSCQN MARDLVNLPG NLLQAEDISA AAVESGKRCG FEVNVFGKEE IEALGMGGLL 

       250        260        270        280        290        300 
AVNRGSQHPP TFTVLDYKPE GKVAKTVALV GKGVTFDSGG ISLKPSEGMG EMKSDMSGAA 

       310        320        330        340        350        360 
SVIGAVEAVA RLGLPIRVIG LIPATDNMPS GSATKPGDVI TTYSGITVEV GNTDAEGRLI 

       370        380        390        400        410        420 
LADALTYAKK QYNPDVIIDL ATLTGACIVA LGYTVAGLFS NDDRLADDIF EAGQITGEKV 

       430        440        450        460        470        480 
WRMPLWEEYD EMIKSDVADV SNLGARGAGS VTASRFLEKF IDGHKKWAHI DIAGPSFSAK 

       490        500 
GAKVSGATGF GVRLLVELLK KWS 

« Hide

References

[1]"Complete sequence of Chlorobium limicola DSM 245."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ovchinnikova G., Zhao F., Li T., Liu Z. expand/collapse author list , Overmann J., Bryant D.A., Richardson P.
Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 245 / NBRC 103803.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001097 Genomic DNA. Translation: ACD90471.1.
RefSeqYP_001943450.1. NC_010803.1.

3D structure databases

ProteinModelPortalB3ED46.
ModBaseSearch...

Protein-protein interaction databases

STRINGB3ED46.

Protein family/group databases

MEROPSM17.003.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6356182.
GenomeReviewsGene locus Clim_1411 in contig CP001097_GR.
KEGGcli:Clim_1411.
PATRIC21374939. VBIChlLim118737_1505.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG742580.
OMASEGMGEM.
ProtClustDBPRK00913.

Family and domain databases

HAMAPMF_00181. Cytosol_peptidase_M17.
[Tree]
InterProIPR011356. Peptidase_M17.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_cytosol_amino.
IPR008283. Peptidase_M17_N.
[Graphical view]
KOK01255.
PANTHERPTHR11963:SF3. Peptidase_M17. 1 hit.
PfamPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSPR00481. LAMNOPPTDASE.
PROSITEPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMPA_CHLL2
AccessionPrimary (citable) accession number: B3ED46
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 22, 2008
Last modified: January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families