B3EBB7 (KATG_GEOLS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Glov_1795

Organism

Geobacter lovleyi (strain ATCC BAA-1151 / DSM 17278 / SZ) [Complete proteome] [HAMAP]

Taxonomic identifier

398767 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	735 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 735	735	Catalase-peroxidase HAMAP MF_01961		PRO_0000354801
Sites
	Active site	96	1	Proton acceptor By similarity
	Metal binding	264	1	Iron (heme axial ligand) By similarity
	Site	92	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	95 ↔ 223		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-249) By similarity
	Cross-link	223 ↔ 249		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-95) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B3EBB7 [UniParc]. Last modified July 22, 2008. Version 1. Checksum: 061AFE9EB6A2BEC4	FASTA	735	80,964
	10 20 30 40 50 60 MSDSKCPVTG KSSRQVAGGG TSNRDWWPNQ LNLQILHQQS TKSNPMGEGF NYRQEFTMLD 70 80 90 100 110 120 LAAVKKDLVA LMTDSQDWWP ADWGHYGGLM IRMAWHSAGT YRMGDGRGGA GSGSQRLAPL 130 140 150 160 170 180 NSWPDNVNLD KARRLLWPVK QKYGRKISWA DLMILAGNCA LESMGFKTFG FGGGREDIWE 190 200 210 220 230 240 PQEDVYWGGE KEWLATSDKP YSRYSGERDL DNPLAAVQMG LIYINPEGPD GNPDPVASGR 250 260 270 280 290 300 DVRETFARMA MNDEETVALV AGGHTFGKCH GAGTADHVGP EPEAAPLEAQ GLGWISSYKS 310 320 330 340 350 360 GKGGDTISSG IEGAWKPNPT TWDMGYLKVL FKYEWELVKS PAGAHQWLAK DVADEDMVVD 370 380 390 400 410 420 AHDPAKKRRP MMTTADLSLR FDPAYEKIAR RYLANPDEFA DAFARAWFKL THRDMGPRSR 430 440 450 460 470 480 YLGPEVPAEE LIWQDPVPAA THELINNSDV ADLKVTILAT GLSISQLVTT AWASASTFRG 490 500 510 520 530 540 SDKRGGANGA RIRLAPQKDW EVNQPGQLAK VLETLAAVQQ EFNAAQSGNK RVSLADLIVL 550 560 570 580 590 600 GGCAAVEQAA RNAGQTVAVP FTPGRTDASQ EQTDTASFEV LEPKADGFRN YQKEKYAVSA 610 620 630 640 650 660 EELLVDRAQL LTLTAPEMTV LIGGMRVLNA NFGNARHGVF TKRPEALTND FFVNLLDMAT 670 680 690 700 710 720 IWKATSEDQD LFEGRDRTSG ELKWTATRVD LIFGSNSQLR AIAEVYGCED SQEKFLQDFA 730 AAWDKVMQAD RFELV

References

[1]

"Complete sequence of chromosome of Geobacter lovleyi SZ." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Meincke L., Brettin T., Detter J.C., Han C., Tapia R., Kuske C.R., Schmutz J., Larimer F. , Land M., Hauser L., Kyrpides N., Mikhailova N., Sung Y., Fletcher K.E., Ritalahti K.M., Loeffler F.E., Richardson P. Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1151 / DSM 17278 / SZ.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001089 Genomic DNA. Translation: ACD95511.1.
RefSeq	YP_001952031.1. NC_010814.1.
3D structure databases
ProteinModelPortal	B3EBB7.
SMR	B3EBB7. Positions 20-734.
ModBase	Search...
Protein-protein interaction databases
STRING	B3EBB7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6366033.
GenomeReviews	Gene locus Glov_1795 in contig CP001089_GR.
KEGG	glo:Glov_1795.
PATRIC	21995263. VBIGeoLov31523_1737.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285610.
OMA	WPNALNL.
ProtClustDB	PRK15061.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_GEOLS

Accession

Primary (citable) accession number: B3EBB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	July 22, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program